Human bronchial epithelial cells modulate CD3 and mitogen-induced DNA synthesis in T cells but function poorly as antigen-presenting cells compared to pulmonary macrophages

• Published in: Journal of allergy and clinical immunology Vol. 87; no. 5; pp. 930 - 938
• Main Authors: Mezzetti, Maurizio, Soloperto, Massimo, Fasoli, Angelo, Mattoli, Sabrina
• Format: Journal Article
• Language: English
• Published: New York, NY Mosby, Inc 01.05.1991; Elsevier
• Subjects: Analysis of the immune response. Humoral and cellular immunity; Antigen-Presenting Cells - immunology; Antigen-Presenting Cells - metabolism; Antigens, CD - immunology; Antigens, Differentiation, T-Lymphocyte - immunology; Biological and medical sciences; Bronchi - cytology; Bronchi - immunology; CD3 Complex; Cell Division - immunology; Cell Separation; Cells, Cultured - immunology; Cells, Cultured - metabolism; DNA - biosynthesis; DNA - drug effects; Epithelial Cells; Epithelium - immunology; Fundamental and applied biological sciences. Psychology; Fundamental immunology; HLA-DR Antigens - analysis; Humans; Immunobiology; Lung - cytology; Lung - immunology; Macrophages - immunology; Mitogens - pharmacology; Organs and cells involved in the immune response; Receptors, Antigen, T-Cell - immunology; T-Lymphocytes - immunology; T-Lymphocytes - metabolism; Ab; IL-1; Ag; FCS; PGE 2; Ia; PHA; MHC; IL-2; EC; INF-γ; IL-6; Human; Cell culture; Antigen presentation; HLA-System; Major histocompatibility system; Class II histocompatibility antigen; Bronchus; Accessory cell; Biosynthesis; Respiratory system; DNA; T-Lymphocyte; Epithelial cell
• ISSN: 0091-6749
• DOI: 10.1016/0091-6749(91)90414-J

This study was undertaken to investigate the ability of bronchial epithelial cells (ECs) to function as accessory cells. Pulmonary monocytes were our reference cells. ECs and pulmonary monocytes were isolated from the bronchial mucosa and pulmonary parenchyma of subjects undergoing lobectomy for standard clinical reasons. Circulating autologous T cells were rigorously depleted of accessory cells to the extent that they lost the capacity to respond to mitogenic lectins alone. ECs restored mitogen-induced DNA synthesis and DNA synthesis triggered by CD3 cross-linking in T cells, as did pulmonary macrophages, and this was unrelated to HLA-DR expression. The ability of promoting T cell proliferation after CD3 cross-linking was, in part, due to the secretory products of ECs, since their supernatants were also effective. Interferon-γ-treated ECs were capable of presenting antigens to autologous T cells. This was an HLA-DR-restricted phenomenon, but EC efficiency in this system was <40% of efficiency demonstrated by pulmonary monocytes. However, ECs greatly upregulated antigen-specific responses supported by pulmonary monocytes.