Recognition of the DNA helix stabilizing anthramycin-N2 guanine adduct by UVRABC nuclease

• Published in: Journal of molecular biology Vol. 203; no. 4; pp. 939 - 947
• Main Authors: Walter, Ronald B., Pierce, James, Case, Roger, Tang, Moon-shong
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 20.10.1988; Elsevier
• Subjects: Analytical, structural and metabolic biochemistry; Anthramycin - metabolism; Base Sequence; Benzodiazepinones - metabolism; Biological and medical sciences; DNA, Bacterial - genetics; Dna, deoxyribonucleoproteins; Endodeoxyribonucleases - metabolism; Escherichia coli Proteins; Exodeoxyribonucleases - metabolism; Fundamental and applied biological sciences. Psychology; Guanine - metabolism; Models, Genetic; Molecular Sequence Data; Nucleic acids; Plasmids; Viral Proteins; ExoIII; Atm; u.v; λ-exo; bp; Antineoplastic agent; Molecular structure; Stabilization; DNA; Molecular interaction; Nuclease; Recognition

The binding of the anti-tumor antibiotic anthramycin to a defined linear DNA fragment was investigated using both exonuclease III and lambda exonuclease. We show that most of the guanine residues are reactive toward anthramycin; however, several guanine residues showed preferential reactivity for th...