Development of hexachlorobenzene porphyria in rats: Time sequence and relationship with lipid peroxidation

• Published in: Food and chemical toxicology Vol. 27; no. 5; pp. 317 - 321
• Main Authors: Visser, O., van den Berg, J.W.O., Edixhoven-Bosdijk, A., Koole-Lesuis, R., Rietveld, T., Wilson, J.H.P.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 1989; New York, NY Elsevier Science
• Subjects: Animals; Biological and medical sciences; Chemical and industrial products toxicology. Toxic occupational diseases; Chlorobenzenes - toxicity; Fatty Acids - analysis; Female; Free Radicals; Hexachlorobenzene - toxicity; Lipid Peroxidation; Liver - analysis; Liver - enzymology; Medical sciences; Microsomes, Liver - analysis; Porphyrias - chemically induced; Porphyrias - etiology; Porphyrins - analysis; Porphyrins - urine; Rats; Rats, Inbred Strains; Time Factors; Toxicology; Uroporphyrinogen Decarboxylase - analysis; Various organic compounds; MDA; PHA = polyhalogenated aromatic; G6Pase; PUFA; URO-D; HCB; Porphyria; Hydrocarbon; Organochlorine compounds; Porphyrin; Toxicity; Liver; Polycyclic aromatic compound; Free radical
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/0278-6915(89)90134-8

The relationship between the development of porphyria and free-radical formation induced by hexachlorobenzene was studied in iron-overloaded rats. The first sign of porphyria, an increase in porphyrins in the liver, was detected at day 22. Liver malondialdehyde was also increased at day 22. During the following weeks, liver porphyrins and malondialdehyde increased simultaneously, accompanied by a decrease in uroporphyrinogen decarboxylase activity and glucose-6-phosphate activity in liver, and a high excretion of porphyrins in the urine. In the rats given hexachlorobenzene, changes were detected in the pattern of lipids in the liver microsomes. In comparison with the controls, there were decreases in C20:4 and C22:5 fatty acids, whereas the fatty acid C20:3w6 was increased. In this study of hexachlorobenzene-induced liver damage there was no difference in the time course of the development of porphyria and that of lipid peroxidation.