New tester strains with improved bioactivation capacity for the Drosophila wing-spot test

In Drosophila melanogaster new tester strains for the somatic mutation and recombination test (SMART) in the wing were constructed with the aim of increasing the metabolic capacity to activate promutagens. Some aspects of the genetic control of the xenobiotics metabolism in Drosophila are already kn...

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Bibliographic Details
Published inMutation Research/Environmental Mutagenesis and Related Subjects Vol. 216; no. 3; pp. 179 - 187
Main Authors Frölich, A., Würgler, F.E.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.06.1989
Subjects
Animals
Biotransformation
Cytochrome P-450
Diethylnitrosamine
Diethylnitrosamine - toxicity
Dose-Response Relationship, Drug
Drosophila
Drosophila melanogaster - drug effects
Drosophila melanogaster - genetics
Genetic control
Metabolic activation
Mutagenicity Tests - methods
Wings, Animal
Metabolic activation
Genetic control
Drosophila
Diethylnitrosamine
Cytochrome P-450
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Summary:In Drosophila melanogaster new tester strains for the somatic mutation and recombination test (SMART) in the wing were constructed with the aim of increasing the metabolic capacity to activate promutagens. Some aspects of the genetic control of the xenobiotics metabolism in Drosophila are already known. In the DDT-resistant strain Oregon R(R) the RI gene at position 65.0 on chromosome 2 is responsible for the high constitutive expression of cytochrome P-450-dependent activities typical for this strain. Therefore, chromosomes 1 and 2 in the original mwh (multiple wing hairs) and flr 3 (flare 3) tester strains were substituted for chromosomes 1 and 2 from the Oregon R(R) strain. In assays with the model promutagen diethylnitrosamine an increased sensitivity of about 2.5-fold was found for this new set of ‘HB’ strains (‘High Bioactivation (HB) cross’).
ISSN:0165-1161
0027-5107
DOI:10.1016/0165-1161(89)90003-4