Identification of a high-efficiency baculovirus DNA replication origin that functions in insect and mammalian cells

• Published in: Journal of virology Vol. 88; no. 22; pp. 13073 - 13085
• Main Authors: Wu, Yueh-Lung, Wu, Carol-P, Huang, Yu-Hui, Huang, Sheng-Ping, Lo, Huei-Ru, Chang, Hao-Shuo, Lin, Pi-Hsiu, Wu, Ming-Cheng, Chang, Chia-Jung, Chao, Yu-Chan
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01.11.2014
• Subjects: Animals; Autographa californica; Baculoviridae - genetics; Baculovirus; Cell Line; DNA Mutational Analysis; DNA Replication; Genome and Regulation of Viral Gene Expression; Insecta; Mammals; Nuclear polyhedrosis virus; Replication Origin; Sequence Deletion; Spodoptera frugiperda

The p143 gene from Autographa californica multinucleocapsid nucleopolyhedrovirus (AcMNPV) has been found to increase the expression of luciferase, which is driven by the polyhedrin gene promoter, in a plasmid with virus coinfection. Further study indicated that this is due to the presence of a repli...