Real-time monitoring of drug-induced changes in the stomach acidity of living rats using improved pH-sensitive nitroxides and low-field EPR techniques

• Published in: Journal of magnetic resonance (1997) Vol. 182; no. 1; pp. 1 - 11
• Main Authors: Potapenko, Dmitrii I., Foster, Margaret A., Lurie, David J., Kirilyuk, Igor A., Hutchison, James M.S., Grigor’ev, Igor A., Bagryanskaya, Elena G., Khramtsov, Valery V.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.09.2006
• Subjects: Animals; Cyclic N-Oxides - chemistry; Cyclic N-Oxides - metabolism; Electron Spin Resonance Spectroscopy - methods; Gastric Acid - metabolism; Hydrogen-Ion Concentration; Imidazolidines - metabolism; In vivo EPR techniques; In vivo pH measurement; Male; Molecular Structure; Nitroxide; Rats; Rats, Sprague-Dawley; Spin Labels; Stomach - metabolism; Stomach acidity; Stomach acidity; In vivo pH measurement; Nitroxide; In vivo EPR techniques

New improved pH-sensitive nitroxides were applied for in vivo studies. An increased stability of the probes towards reduction was achieved by the introduction of the bulky ethyl groups in the vicinity of the paramagnetic N O fragment. In addition, the range of pH sensitivity of the approach was exte...