Development, comparison, and validation using ELISAs for the determination of domoic acid in California sea lion body fluids

• Published in: Journal of AOAC International Vol. 97; no. 2; pp. 345 - 355
• Main Authors: Seubert, Erica L, Howard, Meredith D A, Kudela, Raphael M, Stewart, Thomas N, Litaker, R Wayne, Evans, Richard, Caron, David A
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.03.2014
• Subjects: Animals; Antibodies; Body fluids; Body Fluids - chemistry; Chemical properties; Chromatography, Liquid - methods; Enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - methods; Enzyme-Linked Immunosorbent Assay - veterinary; Identification and classification; Kainic Acid - analogs & derivatives; Kainic Acid - chemistry; Mass Spectrometry; Methods; Neuromuscular Depolarizing Agents - chemistry; Physiological aspects; Reproducibility of Results; Sea Lions; United States

Mortalities of California sea lions (Zalophus californianus) attributed to the neurotoxin domoic acid (DA) produced by the diatom Pseudo-nitzschia have occurred repeatedly along the U.S. west coast since the late 1990s. Quantifying the amount of DA in these animals and correlating this information w...