Homologous ELISA for detection of oligomeric human TNF: properties of the assay

• Published in: Journal of immunological methods Vol. 186; no. 2; pp. 161 - 170
• Main Authors: Petyovka, Natalia, Lyach, Lyudmila, Voitenok, Nikolai N.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 26.10.1995; Elsevier
• Subjects: Analysis of the immune response. Humoral and cellular immunity; Animals; Antibodies, Monoclonal - immunology; Antibody Specificity; Antigen-Antibody Reactions; Biological and medical sciences; Biological Assay; Cells, Cultured; Culture Media, Conditioned - analysis; Detergents - pharmacology; ELISA; Enzyme-Linked Immunosorbent Assay; False Positive Reactions; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Humans; IL-8; Immunobiology; Interleukin-8 - analysis; Kinetics; L Cells (Cell Line); Lymphokines, interleukins ( function, expression); Lymphotoxin-alpha - analysis; Mice; Monoclonal antibody; Monocytes - metabolism; Neutralization Tests; Oligomeric form; Polysorbates - pharmacology; Protein Conformation; Quaternary structure; Recombinant Proteins - analysis; Recombinant Proteins - immunology; Regulatory factors and their cellular receptors; Sensitivity and Specificity; Tumor necrosis factor; Tumor Necrosis Factor-alpha - analysis; Tumor Necrosis Factor-alpha - chemistry; PBS; Tumor necrosis factor; Oligomeric form; Quaternary structure; MoAb; Monoclonal antibody; IL-8; TNF; Hm-ELISA; Ht-ELISA; ELISA; Human; Investigation method; Cytokine; Detection; Oligomer; ELISA assay; Interleukin 8

In order to quantify oligomeric human tumor necrosis factor-α (TNF), we have developed a sensitive homologous enzyme-linked immunosorbent assay (Hm-ELISA) using the same monoclonal antibody (MoAb) for both solid and liquid phase. Different anti-TNF MoAb have been compared in terms of their efficacy...