In vivo Imaging of Tumor Transduced with Bimodal Lentiviral Vector Encoding Human Ferritin and Green Fluorescent Protein on a 1.5T Clinical Magnetic Resonance Scanner

• Published in: Cancer research (Chicago, Ill.) Vol. 70; no. 18; pp. 7315 - 7324
• Main Authors: Kim, Hoe Suk, Cho, Hye Rim, Choi, Seung Hong, Woo, Ji Su, Moon, Woo Kyung
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA American Association for Cancer Research 15.09.2010
• Subjects: Animals; Antineoplastic agents; Apoferritins - biosynthesis; Apoferritins - genetics; Biological and medical sciences; Breast Neoplasms - genetics; Breast Neoplasms - metabolism; Breast Neoplasms - pathology; Cell Line, Tumor; Genetic Vectors - genetics; Glioma - genetics; Glioma - metabolism; Glioma - pathology; Green Fluorescent Proteins - biosynthesis; Green Fluorescent Proteins - genetics; Humans; Lentivirus - genetics; Magnetic Resonance Imaging - instrumentation; Magnetic Resonance Imaging - methods; Medical sciences; Mice; Mice, Inbred BALB C; Mice, Nude; Pharmacology. Drug treatments; Proto-Oncogene Proteins c-myc - biosynthesis; Proto-Oncogene Proteins c-myc - genetics; Rats; Rats, Inbred F344; Recombinant Fusion Proteins - biosynthesis; Recombinant Fusion Proteins - genetics; Transduction, Genetic; Tumors; Human; Scanner; Magnetic resonance; Ferritin; Retroviridae; Malignant tumor; Nuclear magnetic resonance imaging; Lentivirus; Virus; In vivo; Signal transduction; Imaging; Green fluorescent protein; Medical imagery; Vector; Cancer

A combination of reporter genes for magnetic resonance imaging (MRI) and optical imaging can provide an additional level of noninvasive and quantitative information about biological processes occurring in deep tissues. We developed a bimodal lentiviral vector to monitor deep tissue events using MRI...