Aging and presbycusis: Effects on 2-deoxy- d-glucose uptake in the mouse auditory brain stem in quiet

• Published in: Experimental neurology Vol. 99; no. 3; pp. 615 - 621
• Main Authors: Willott, James F., Hunter, Kelly Paris, Coleman, James R.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 01.03.1988; Elsevier
• Subjects: Aging - metabolism; Animals; Autoradiography; Biological and medical sciences; Brain Stem - metabolism; Glucose - metabolism; Hearing Loss, Sensorineural - metabolism; Inferior Colliculi - metabolism; Medical sciences; Mice; Mice, Inbred C57BL; Mice, Inbred CBA; Nervous system involvement in other diseases. Miscellaneous; Neurology; Presbycusis - metabolism; Trigeminal Nuclei - metabolism; mo; IC; TN; 2DG; AVCN; Radionuclide study; Nervous system diseases; Brain stem; Rodentia; Hereditary; Metabolism; Uptake; Pathology; Autoradiography; Vertebrata; Experimental disease; Mammalia; Mouse; Presbyacusia; ENT disease; Age
• ISSN: 0014-4886; 1090-2430
• DOI: 10.1016/0014-4886(88)90178-1

Autoradiography was used to assess the incorporation of [2- 14C]deoxy- d-glucose by the auditory brain stem of young and aging mice of the C57BL 6 strain (which demonstrates progressive chronic sensorineural hearing loss) and the CBA strain (which maintains good hearing until late in life). Animals were injected with labeled 2-deoxyglucose and placed in quiet for 45 min; brain stem sections were prepared for autoradiography. The amounts of 2-deoxyglucose incorporated into the anterior ventral cochlear nucleus (AVCN), inferior colliculus (IC), and trigeminal nerve (TN) were densitometrically analyzed. Within each subject, the densities of the three structures were statistically compared. In every mouse, inferior colliculus density was greater than that of the anterior ventral cochlear nucleus, which was greater than trigeminal nerve density. To compare subject groups, relative densities (inferior colliculus and anterior ventral cochlear nucleus re: trigeminal nerve) were used; no significant differences were found between groups. Thus, aging, with or without severe loss of hearing, is not associated with altered incorporation of 2-deoxyglucose (and presumably glucose) in quiet.