The Naturally Occurring Flavolignan, Deoxypodophyllotoxin, Inhibits Lipopolysaccharide-Induced iNOS Expression through the NF-κB Activation in RAW264.7 Macrophage Cells

Deoxypodophyllotoxin (DPT), a naturally occurring flavolignan with anti-inflammatory activity, was isolated from Anthriscus sylvestris HOFFM., and we examined its effects on the expression of inducible nitric oxide synthase (iNOS) in lipopolysaccharide (LPS)-stimulated, murine macrophage-like RAW264...

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Published inBiological & pharmaceutical bulletin Vol. 31; no. 7; pp. 1312 - 1315
Main Authors Jin, Meihua, Moon, Tae Chul, Quan, Zhejiu, Lee, Eunkyung, Kim, Yun Kyung, Yang, Ju Hae, Suh, Seok-Jong, Jeong, Tae Cheon, Lee, Seung Ho, Kim, Cheorl-Ho, Chang, Hyeun Wook
Format Journal Article
LanguageEnglish
Published The Pharmaceutical Society of Japan 01.07.2008
Subjects
deoxypodophyllotoxin
electrophoretic mobility shift analysis
inducible nitric oxide synthase
nuclear factor-κB
pyrrolidinedithiocarbamate
RAW264.7 cell
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Abstract Deoxypodophyllotoxin (DPT), a naturally occurring flavolignan with anti-inflammatory activity, was isolated from Anthriscus sylvestris HOFFM., and we examined its effects on the expression of inducible nitric oxide synthase (iNOS) in lipopolysaccharide (LPS)-stimulated, murine macrophage-like RAW264.7 cells. Western blot analysis performed with specific anti-iNOS antibodies showed that a decrease in nitric oxide (NO) was accompanied by a decrease in the iNOS protein level. To clarify the mechanistic basis for DPT's ability to inhibit iNOS induction, we examined the effect of DPT on nuclear factor (NF)-κB transcriptional activity and DNA binding activity. DPT potently suppressed both reporter gene activity and DNA binding activity. These findings suggest that DPT in RAW264.7 cells abolished LPS-induced iNOS expression by inhibiting the transcription factor, NF-κB.
AbstractList Deoxypodophyllotoxin (DPT), a naturally occurring flavolignan with anti-inflammatory activity, was isolated from Anthriscus sylvestris HOFFM., and we examined its effects on the expression of inducible nitric oxide synthase (iNOS) in lipopolysaccharide (LPS)-stimulated, murine macrophage-like RAW264.7 cells. Western blot analysis performed with specific anti-iNOS antibodies showed that a decrease in nitric oxide (NO) was accompanied by a decrease in the iNOS protein level. To clarify the mechanistic basis for DPT's ability to inhibit iNOS induction, we examined the effect of DPT on nuclear factor (NF)-κB transcriptional activity and DNA binding activity. DPT potently suppressed both reporter gene activity and DNA binding activity. These findings suggest that DPT in RAW264.7 cells abolished LPS-induced iNOS expression by inhibiting the transcription factor, NF-κB.
Author Moon, Tae Chul
Kim, Yun Kyung
Suh, Seok-Jong
Lee, Seung Ho
Jin, Meihua
Jeong, Tae Cheon
Quan, Zhejiu
Kim, Cheorl-Ho
Lee, Eunkyung
Chang, Hyeun Wook
Yang, Ju Hae
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  fullname: Quan, Zhejiu
  organization: College of Pharmacy, Yeungnam University
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  fullname: Lee, Eunkyung
  organization: Daegu Gyeongbuk Institute for Oriental Medicine Industry
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  organization: College of Pharmacy, Yeungnam University
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  organization: College of Pharmacy, Yeungnam University
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  fullname: Suh, Seok-Jong
  organization: Department of Biological Science, Sungkyunkwan University
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  organization: College of Pharmacy, Yeungnam University
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  fullname: Lee, Seung Ho
  organization: College of Pharmacy, Yeungnam University
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  fullname: Kim, Cheorl-Ho
  organization: Department of Biological Science, Sungkyunkwan University
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  fullname: Chang, Hyeun Wook
  organization: College of Pharmacy, Yeungnam University
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Snippet Deoxypodophyllotoxin (DPT), a naturally occurring flavolignan with anti-inflammatory activity, was isolated from Anthriscus sylvestris HOFFM., and we examined...
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SubjectTerms deoxypodophyllotoxin
electrophoretic mobility shift analysis
inducible nitric oxide synthase
nuclear factor-κB
pyrrolidinedithiocarbamate
RAW264.7 cell
Title The Naturally Occurring Flavolignan, Deoxypodophyllotoxin, Inhibits Lipopolysaccharide-Induced iNOS Expression through the NF-κB Activation in RAW264.7 Macrophage Cells
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