Comparison of three methods for competitive binding of monoclonal antibodies The localization of antigenic sites for monoclonal antibodies on Panulirus interruptus hemocyanin
The competitive binding of a panel of monoclonal antibodies against hemocyanin of Panulirus interruptus hemocyanin was investigated with three different methods. A competitive-binding immunoassay method was more successful in the determination of ternary complexes than gel electrophoresis and gel fi...
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| Published in | Journal of immunological methods Vol. 190; no. 1; pp. 117 - 125 |
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| Main Authors | , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Amsterdam
Elsevier B.V
28.03.1996
Elsevier |
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| Abstract | The competitive binding of a panel of monoclonal antibodies against hemocyanin of
Panulirus interruptus hemocyanin was investigated with three different methods. A competitive-binding immunoassay method was more successful in the determination of ternary complexes than gel electrophoresis and gel filtration. The latter two methods could only be applied with antibodies possessing a higher affinity. Eleven monoclonal antibodies were assigned to groups on the basis of their interactions with five antigenic regions. |
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| AbstractList | The competitive binding of a panel of monoclonal antibodies against hemocyanin of
Panulirus interruptus hemocyanin was investigated with three different methods. A competitive-binding immunoassay method was more successful in the determination of ternary complexes than gel electrophoresis and gel filtration. The latter two methods could only be applied with antibodies possessing a higher affinity. Eleven monoclonal antibodies were assigned to groups on the basis of their interactions with five antigenic regions. The competitive binding of a panel of monoclonal antibodies against hemocyanin of Panulirus interruptus hemocyanin was investigated with three different methods. A competitive-binding immunoassay method was more successful in the determination of ternary complexes than gel electrophoresis and gel filtration. The latter two methods could only be applied with antibodies possessing a higher affinity. Eleven monoclonal antibodies were assigned to groups on the basis of their interactions with five antigenic regions. |
| Author | Perton, Frank G. Smilda, Tim van Uffelen, Bart Erik Dijkema, Jan H. Beintema, Jaap J. |
| Author_xml | – sequence: 1 givenname: Frank G. surname: Perton fullname: Perton, Frank G. – sequence: 2 givenname: Jan H. surname: Dijkema fullname: Dijkema, Jan H. – sequence: 3 givenname: Tim surname: Smilda fullname: Smilda, Tim – sequence: 4 givenname: Bart Erik surname: van Uffelen fullname: van Uffelen, Bart Erik – sequence: 5 givenname: Jaap J. surname: Beintema fullname: Beintema, Jaap J. |
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| Keywords | PBS BSA Epitope Hemocyanin Fab Competitive binding Panulirus interruptus Antigenic region Antigenic determinant Monoclonal antibody Method Macrura Crustacea Competitive fixation Arthropoda Decapoda Invertebrata Cuproprotein Comparative study |
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Biol. doi: 10.1016/1047-8477(90)90087-S contributor: fullname: Lamy – start-page: 44 year: 1974 ident: 10.1016/0022-1759(95)00258-8_BIB10 article-title: Disc Electrophoresis and Related Techniques of Polyacrylamide Gel Electrophoresis contributor: fullname: Maurer |
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Panulirus interruptus hemocyanin was investigated with three different... The competitive binding of a panel of monoclonal antibodies against hemocyanin of Panulirus interruptus hemocyanin was investigated with three different... |
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| SubjectTerms | Animals Antibodies, Monoclonal - immunology Antibody Specificity Antigenic region Binding Sites, Antibody Binding, Competitive Biological and medical sciences Chromatography, Gel Competitive binding Crustacea - immunology Electrophoresis, Polyacrylamide Gel Epitope Epitope Mapping - methods Fundamental and applied biological sciences. Psychology Fundamental immunology Hemocyanin Hemocyanins - chemistry Hemocyanins - immunology Molecular immunology Panulirus interruptus Techniques |
| Title | Comparison of three methods for competitive binding of monoclonal antibodies The localization of antigenic sites for monoclonal antibodies on Panulirus interruptus hemocyanin |
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