Automated Screening Procedure for High-Throughput Generation of Antibody Fragments

• Published in: BioTechniques Vol. 33; no. S6; pp. S30 - S37
• Main Authors: Hallborn, Johan, Carlsson, Roland
• Format: Journal Article
• Language: English
• Published: England Future Science Ltd 01.12.2002
• Subjects: Antigens - analysis; Antigens - chemistry; Antigens - genetics; Antigens - metabolism; Combinatorial Chemistry Techniques - instrumentation; Combinatorial Chemistry Techniques - methods; Enzyme-Linked Immunosorbent Assay - instrumentation; Enzyme-Linked Immunosorbent Assay - methods; Gene Expression Regulation; Immunoglobulin Fragments - chemistry; Immunoglobulin Fragments - genetics; Immunoglobulin Fragments - metabolism; Protein Array Analysis - instrumentation; Protein Array Analysis - methods; Proteomics - instrumentation; Proteomics - methods; Robotics - instrumentation; Robotics - methods
• ISSN: 0736-6205; 1940-9818
• DOI: 10.2144/dec02-hallborn

In the emerging field of proteomics, there is an urgent need for catcher molecules such as antibodies for detecting the proteome or parts of the proteome in a microarray format. A suitable source for providing a large diversity of binders is obtained by combinatorial libraries, such as phage display libraries of single chain antibody fragments (scFv) or Fab fragments. To find novel binders from the n-CoDeR libraries with a high throughput, we have automated the screening process with robotics. The automated system is configured to screen tens of thousands of clones per day to target antigens in various formats, including peptides and soluble proteins, as well as cell-bound targets; thus, it is well designed to meet demands from the proteomics area.