Cytokine-gene expression in measles-infected adult human glial cells

• Published in: Journal of neuroimmunology Vol. 49; no. 1; pp. 171 - 179
• Main Authors: Yamabe, Toshio, Dhir, Gita, Cowan, Elliot P., Wolf, Aizik L., Bergey, Gregory K., Krumholz, Allan, Barry, Elizabeth, Hoffman, Paul M., Dhib-Jalbut, Suhayl
• Format: Journal Article
• Language: English
• Published: London Elsevier B.V 1994; Amsterdam Elsevier; New York, NY
• Subjects: Adolescent; Adult; Aged; Base Sequence; Biological and medical sciences; Cells, Cultured; Cytokines - genetics; Female; Gene Expression; Human viral diseases; Humans; Infectious diseases; Interleukin-1 - genetics; Interleukin-1β; Interleukin-6; Interleukin-6 - genetics; Male; Measles virus; Measles virus - pathogenicity; Medical sciences; Microglia; Microglia - metabolism; Molecular Sequence Data; Oligodendroglia - metabolism; Tumor necrosis factor α; Tumor Necrosis Factor-alpha - genetics; Viral diseases; Viral diseases with cutaneous or mucosal lesions and viral diseases of the eye; Interleukin-1β; Tumor necrosis factor α; Microglia; Interleukin-6; Measles virus; Human; Skin disease; Cytokine; Gene expression; Paramyxoviridae; Infection; Virus; Gene; Viral disease; Measles; Morbillivirus; Infected cell
• ISSN: 0165-5728; 1872-8421
• DOI: 10.1016/0165-5728(94)90193-7

The expression of interleukin (IL)-1β, IL-6 and tumor necrosis factor (TNF) α transcripts in cultured human glial cells was examined using reverse transcription followed by polymerase chain reaction (PCR) amplification and Southern blot quantitation. Microglial cultures derived from brain biopsy specimens from three different individuals expressed transcripts for the three cytokines under basal culture conditions. This expression was enhanced in response to measles virus (MV) infection (IL-1β, 2.2–8.8-fold; IL-6,2.5–8.4-fold; TNFα, 2.2–3.2-fold). Neither IL-1β nor TNFα transcripts were detectable in undissociated brain tissue from two individuals, suggesting that the basal expression of these cytokines in culture may have been induced by tissue dissociation or by the culture conditions. Oligodendrocytes did not express cytokine transcripts under basal culture conditions, and IL-1β and IL-6 but not TNFα transcripts could be induced by MV. Similarly, meningeal fibroblasts expressed IL-1β and IL-6 but not TNFα in response to MV-infection, suggesting that the production of TNFα is more cell type-restricted than either IL-1β or IL-6. The results indicate that adult human microglia can participate in the inflammatory response to MV infection in the CNS by producing cytokines that contribute to inflammation and demyelination. In addition, besides their role in myelination, oligodendrocytes can potentially influence immunoreactivity in the CNS by producing IL-1β and IL-6.