A new ELISA for the detection of anti-heparan sulfate reactivity, using photobiotinylated antigen

• Published in: Journal of immunological methods Vol. 176; no. 1; pp. 33 - 43
• Main Authors: Hylkema, M.N., Kramers, C., Van der Wal, T.J., Van Bruggen, M.C.J., Swaak, A.J.G., Berden, J.H.M., Smeenk, R.J.T.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 10.11.1994; Elsevier
• Subjects: Animals; Anti-HS reactivity; Antibodies, Antinuclear - analysis; Antibodies, Monoclonal; Autoantibodies - analysis; Autoimmunity (experimental aspects and models); Biological and medical sciences; Biotin; Cross Reactions - immunology; Dermatology; DNA - immunology; ELISA; Enzyme-Linked Immunosorbent Assay - methods; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Glomerulonephritis; Heparitin Sulfate - immunology; Humans; Lupus Erythematosus, Systemic - diagnosis; Lupus Erythematosus, Systemic - immunology; Lupus Nephritis - diagnosis; Lupus Nephritis - immunology; Medical sciences; Mice; Nephritis; Nephrology. Urinary tract diseases; Nephropathies. Renovascular diseases. Renal failure; Systemic lupus erythematous; Vascular disorders of the skin; PBS; Nephritis; PT; HRP; Anti-HS reactivity; TMB; HS; SLE; mAb; Systemic lupus erythematous; BSA; GBM; FCS; NGS; ELISA; Human; Skin disease; Autoantigen; Nephropathy; Protamine; Autoantibody; Heparitin sulfate; Lupus erythematosus; Autoimmune disease; ELISA assay

Autoantibodies reacting with a great variety of autoantigens are characteristic for the autoimmune disease systemic lupus erythematosus (SLE). Although reactivity with heparan sulfate (HS) in sera of patients with SLE is found in association with the occurrence of nephritis, the aetiological signifi...