Separation and purification of S49 mouse lymphoma histones by reversed-phase high-performance liquid chromatography

• Published in: Analytical biochemistry Vol. 163; no. 2; pp. 427 - 432
• Main Authors: McCroskey, Mark C., Groppi, Vincent E., Pearson, James D.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.06.1987; Elsevier
• Subjects: Animals; Applied sciences; Chromatography, High Pressure Liquid - methods; Exact sciences and technology; Fluorocarbons; high-performance liquid chromatography; histones; Histones - classification; Histones - isolation & purification; HPLC; lymphoma; Lymphoma - analysis; Mice; Other techniques and industries; protein purification; proteins; protein purification; histones; proteins; HPLC; lymphoma

A rapid reversed-phase high-performance liquid chromatography procedure for the fractionation of histones from S49 mouse lymphoma cells is reported. The system utilizes a Vydac C 4 macroporous column, heptafluorobutyric acid as solubilizing and ion-pairing agent, and an acetonitrile gradient. All fi...