Separation and purification of S49 mouse lymphoma histones by reversed-phase high-performance liquid chromatography

A rapid reversed-phase high-performance liquid chromatography procedure for the fractionation of histones from S49 mouse lymphoma cells is reported. The system utilizes a Vydac C 4 macroporous column, heptafluorobutyric acid as solubilizing and ion-pairing agent, and an acetonitrile gradient. All fi...

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Bibliographic Details
Published inAnalytical biochemistry Vol. 163; no. 2; pp. 427 - 432
Main Authors McCroskey, Mark C., Groppi, Vincent E., Pearson, James D.
Format Journal Article
LanguageEnglish
Published San Diego, CA Elsevier Inc 01.06.1987
Elsevier
Subjects
Animals
Applied sciences
Chromatography, High Pressure Liquid - methods
Exact sciences and technology
Fluorocarbons
high-performance liquid chromatography
histones
Histones - classification
Histones - isolation & purification
HPLC
lymphoma
Lymphoma - analysis
Mice
Other techniques and industries
protein purification
proteins
protein purification
histones
proteins
HPLC
lymphoma
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Summary:A rapid reversed-phase high-performance liquid chromatography procedure for the fractionation of histones from S49 mouse lymphoma cells is reported. The system utilizes a Vydac C 4 macroporous column, heptafluorobutyric acid as solubilizing and ion-pairing agent, and an acetonitrile gradient. All five histone classes and several subclass species are separated, including two H1 species, H2B, two H2A species, H4, and two H3 species. Analytical to multimilligram semipreparative scale fractionations are demonstrated while maintaining resolution of all histone types.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(87)90244-2