Construction of Canine Herpesvirus Vector Expressing Foreign Genes Using a lacZ-TK Gene Cassette as a Double Selectional Marker

• Published in: Virus genes Vol. 17; no. 1; pp. 25 - 32
• Main Authors: Xuan, Xuenan, Nishikawa, Yoshifumi, Takashima, Yasuhiro, Tuchiya, Kotaro, Ueda, Susumu, Yokoyama, Naoaki, Maeda, Ken, Mikami, Takeshi, Otsuka, Haruki
• Format: Journal Article
• Language: English
• Published: United States Springer Nature B.V 01.01.1998
• Subjects: Animals; Biomarkers; Cell Line; Cloning, Molecular; DNA, Viral - analysis; DNA, Viral - genetics; Dogs; Gene Expression Regulation, Viral; Genetic Vectors - genetics; Herpesvirus 1, Canid - genetics; Lac Operon - genetics; Lyssavirus; Plaques; Rabies; Recombinant Fusion Proteins - analysis; Recombinant Fusion Proteins - genetics; Recombinants; Thymidine Kinase - genetics; TK gene; Transgenes - genetics; Viruses

An improved method for constructing canine herpesvirus (CHV) recombinants expressing foreign genes by using the lacZ-TK gene cassette as a double selectional marker was developed. A recombinant CHV carrying the lacZ-TK gene at a targeted gene locus was constructed and used as a parental virus for ge...