Cloning and sequencing of mouse VCAM-1 cDNA

• Published in: Gene Vol. 126; no. 2; pp. 261 - 264
• Main Authors: Araki, Masatake, Araki, Kimi, Vassalli, Pierre
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 30.04.1993; Amsterdam Elsevier; New York, NY
• Subjects: adhesion molecules; Amino Acid Sequence; amino acid sequence homology; Animals; Base Sequence; Biological and medical sciences; Blotting, Northern; Cell Adhesion Molecules - genetics; Cloning, Molecular; DNA; Endothelial cells; Fundamental and applied biological sciences. Psychology; Genes. Genome; Humans; immunoglobulin gene superfamily; Mice; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Polymerase Chain Reaction; Sequence Homology, Nucleic Acid; Vascular Cell Adhesion Molecule-1; aa; MRL/1 (mouse strain); IL-1; nt; h; CP; m; TNF; Endothelial cells; bp; CAM; LPS; VCAM-1; amino acid sequence homology; adhesion molecules; RT-PCR; TM; immunoglobulin gene superfamily; SP; HUVECs; Ig; Human; Vertebrata; Mammalia; Complementary DNA; Mouse; Nucleotide sequence; Rodentia; Cell adhesion molecule; Homology; Molecular cloning; Aminoacid sequence
• DOI: 10.1016/0378-1119(93)90377-F
• ISSN: 0378-1119

We report here the nucleotide sequence of a cDNA encoding the mouse vascular cell adhesion molecule-1 (VCAM- 1), a member of the immunoglobulin gene superfamily. The 2972-bp cDNA encodes 739 amino acids (aa) and shows 76.4% and 75.9% similarity with the human VCAM-1 nucleotide (nt) and aa sequence, respectively. Three independent cDNA clones had a 7-Ig unit structure (molecule containing a seven ‘immunoglobulin motif’ repeat), and only sequences corresponding to the 7- (and not to the 6)-Ig unit VCAM were detected by reverse transcription polymerase chain reaction (RT-PCR), indicating that in the mouse this is the dominant, if not exclusive, form of this molecule. Unlike other adhesion molecules, the cytoplasmic tails (19 aa) of mouse and human VCAM-1 are identical, despite a 85.2% similarity only at the nt level, suggesting a critical functional role.