Homologous somatotropin radioreceptor assay utilizing recombinant bovine growth hormone
A homologous radioreceptor assay using recombinant bovine growth hormone and bovine liver membranes is described. The total specific binding of 125I-labeled recombinant bovine growth hormone to the 100000 × g pellet was 48% in 24 h at 25 °C. Hormone binding was partially reversible, with 40% of the...
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| Published in | Molecular and cellular endocrinology Vol. 38; no. 2; pp. 109 - 116 |
|---|---|
| Main Authors | , , |
| Format | Journal Article |
| Language | English |
| Published |
Shannon
Elsevier Ireland Ltd
01.01.1984
Elsevier |
| Subjects | |
| Online Access | Get full text |
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| Abstract | A homologous radioreceptor assay using recombinant bovine growth hormone and bovine liver membranes is described. The total specific binding of
125I-labeled recombinant bovine growth hormone to the 100000 ×
g pellet was 48% in 24 h at 25 °C. Hormone binding was partially reversible, with 40% of the radiolabeled hormone being irreversibly bound. The amount of specific binding varied with assay pH, with the optimum occurring at pH 7.8. Specific binding was temperature-dependent, with greater specific binding occurring at 25°C than at 5°C or 37°C during a 24 h period. Recombinant bovine growth hormone, human growth hormone, ovine growth hormone and recombinant porcine growth hormone competed effectively with
125I-labeled recombinant bovine growth hormone for binding sites, while bovine prolactin and ovine prolactin were needed in amounts 10
6-fold the concentration of recombinant bovine growth hormone to displace the radiolabeled hormone. Surprisingly, human placental lactogen did not displace the radiolabeled hormone. |
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| AbstractList | A homologous radioreceptor assay using recombinant bovine growth hormone and bovine liver membranes is described. The total specific binding of 125I-labeled recombinant bovine growth hormone to the 100 000 X g pellet was 48% in 24 h at 25 degrees C. Hormone binding was partially reversible, with 40% of the radiolabeled hormone being irreversibly bound. The amount of specific binding varied with assay pH, with the optimum occurring at pH 7.8. Specific binding was temperature-dependent, with greater specific binding occurring at 25 degrees C than at 5 degrees C or 37 degrees C during a 24 h period. Recombinant bovine growth hormone, human growth hormone, ovine growth hormone and recombinant porcine growth hormone competed effectively with 125I-labeled recombinant bovine growth hormone for binding sites, while bovine prolactin and ovine prolactin were needed in amounts 10(6)-fold the concentration of recombinant bovine growth hormone to displace the radiolabeled hormone. Surprisingly, human placental lactogen did not displace the radiolabeled hormone. A homologous radioreceptor assay using recombinant bovine growth hormone and bovine liver membranes is described. The total specific binding of 125I-labeled recombinant bovine growth hormone to the 100000 × g pellet was 48% in 24 h at 25 °C. Hormone binding was partially reversible, with 40% of the radiolabeled hormone being irreversibly bound. The amount of specific binding varied with assay pH, with the optimum occurring at pH 7.8. Specific binding was temperature-dependent, with greater specific binding occurring at 25°C than at 5°C or 37°C during a 24 h period. Recombinant bovine growth hormone, human growth hormone, ovine growth hormone and recombinant porcine growth hormone competed effectively with 125I-labeled recombinant bovine growth hormone for binding sites, while bovine prolactin and ovine prolactin were needed in amounts 10 6-fold the concentration of recombinant bovine growth hormone to displace the radiolabeled hormone. Surprisingly, human placental lactogen did not displace the radiolabeled hormone. A homologous radioreceptor assay using recombinant bovine growth hormone and bovine liver membranes is described. The total specific binding of super(125)I-labeled recombinant bovine growth hormone to the 100,000 x g pellet was 48% in 24 h at 25 degree C. Hormone binding was partially reversible, with 40% of the radiolabeled hormone being irreversibly bound. The amount of specific binding varied with assay pH, with the optimum occurring at pH 7.8. Specific binding was temperature-dependent, with greater specific binding occurring at 25 degree C than at 5 degree C or 37 degree C during a 24 h period. |
| Author | Haro, Luis S. Talamantes, Frank J. Collier, Robert J. |
| Author_xml | – sequence: 1 givenname: Luis S. surname: Haro fullname: Haro, Luis S. organization: Biology Board of Studies, Thimann Laboratories, University of California, Santa Cruz, CA 95064, U.S.A – sequence: 2 givenname: Robert J. surname: Collier fullname: Collier, Robert J. organization: Department of Dairy Science, University of Florida, Gainesville, FL 32611 U.S.A – sequence: 3 givenname: Frank J. surname: Talamantes fullname: Talamantes, Frank J. organization: Biology Board of Studies, Thimann Laboratories, University of California, Santa Cruz, CA 95064, U.S.A |
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| Cites_doi | 10.1042/bj1400301 10.1016/S0076-6879(78)52008-9 10.1016/S0021-9258(18)33257-5 10.1016/B978-0-12-153202-4.50012-1 10.1111/j.1399-3011.1983.tb03126.x 10.1210/jcem-37-2-334 10.1210/jcem-28-10-1412 10.1210/endo-112-5-1607 10.1016/S0021-9258(19)52796-X 10.1210/jcem-46-3-374 10.1210/endo-112-6-2032 10.1210/endo-110-5-1758 10.1152/ajplegacy.1931.98.3.511 10.1210/endo-105-2-414 10.1210/jcem-42-3-484 10.1111/j.1399-3011.1983.tb03125.x 10.1210/endo-111-6-1862 10.1530/acta.0.1010574 10.1016/0016-6480(79)90217-X 10.1042/bj2090107 10.1210/endo-98-3-645 10.1210/endo-108-6-2388 10.1080/00288233.1959.10422827 10.1111/j.1365-2265.1982.tb03160.x 10.1016/0026-0495(76)90092-5 10.1016/0005-2795(78)90517-2 10.1007/BF01025354 10.1353/pbm.1982.0025 10.1210/endo-107-6-1954 10.2527/jas1972.354794x 10.1111/j.1399-3011.1982.tb02607.x 10.1016/0014-5793(76)80670-9 10.1042/bj2140885 10.1042/bst0090033 10.1210/endo-111-6-2117 10.1016/S0021-9258(18)50441-5 10.1016/S0021-9258(17)33544-5 10.3168/jds.S0022-0302(83)81857-8 10.1210/endo-112-4-1313 |
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| Keywords | bovine growth hormone growth hormone receptor somatogenic radioreceptor assay homologous hormone-receptor system Protein hormone Vertebrata Mammalia Radioreceptor assay Purification STH Genetic engineering Artiodactyla Beef Ungulata Hormonal receptor |
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| References | Rodbard, Rayford, Cooper, Ross (BIB40) 1968; 28 Shiu, Friesen (BIB43) 1974; 140 Shoer, Shine, Talamantes (BIB44) 1978; 537 Wallis (BIB49) 1978; Vol. 5 Waters, Friesen (BIB51) 1979; 254 Herington, Phillips, Daughaday (BIB20) 1976; 25 Wagner, Veenhuizen (BIB48) 1978; 47 Brumby (BIB5) 1959; 2 Posner (BIB35) 1976; 98 Haeuptle, Aubert, Djiane, Kraehenbuhl (BIB18) 1983; 258 Laron (BIB25) 1982; 18 Bauman, Eisemann, Currie (BIB2) 1982; 41 Robertson, Gillespie, Friesen (BIB39) 1982; 111 Evans, Simpson (BIB12) 1931; 98 Tsushima, Friesen (BIB47) 1973; 37 Pankov, Pozdnyakov (BIB33) 1980; 45 Delfino, Fernandez, Santome (BIB9) 1983; 21 Surowy, Wallis (BIB46) 1983; 2 Fryer (BIB14) 1979; 39 Moore, Jin (BIB30) 1978; 46 Posner, Patel, Vezinhet, Charrier (BIB36) 1980; 107 Schenkman, Cinti (BIB42) 1978; 52 Sasaki, Imai, Tsushima, Matsuzaki (BIB41) 1982; 101 Kyle, Evans, Odell (BIB24) 1982; 169 Hall, Van Wyk (BIB19) 1974; 2 Eakle, Arima, Swanson, Grimek, Bremel (BIB10) 1982; 110 Murthy, Schellenberg, Friesen (BIB31) 1982; 111 Scares, Colosi, Ogren, Talamantes (BIB45) 1983; 112 Peel, Frank, Bauman, Gorewit (BIB34) 1983; 66 Postel-Vinay (BIB37) 1976; 69 Fernandez, Delfino (BIB13) 1983; 209 Gluckman, Butler, Elliott (BIB16) 1983; 112 Yudaev, Pankov (BIB52) 1982; 16 Altszuler (BIB1) 1974; Vol. 4 Wallis, Cadman (BIB50) 1981; 9 Fukushima, Biscoglio de Jimenez Bonino, Cascone, Santomé (BIB15) 1983; 21 Robertson, Friesen (BIB38) 1981; 108 Li, Evans, Simpson (BIB27) 1945; 159 Goodman, Schwartz (BIB17) 1974; Vol. 4 Hughs (BIB22) 1979; 105 Kostyo, Nutting (BIB23) 1974; Vol. 4 Herington, Harrison, Graystone (BIB21) 1983; 112 Lewis, Singh, Tutwiler, Sigel, Vanderlaan, Vanderlaan (BIB26) 1980; 36 Boeynaems, Dumont (BIB3) 1980 Closset, Smal, Gomez, Hennen (BIB8) 1983; 214 Machlin (BIB28) 1972; 35 Eshet, Laron, Pertzelan, Dintzman (BIB11) 1982; 16 Nicoll (BIB32) 1982; 25 Mattera, Dellacha (BIB29) 1982; 19 Carr, Friesen (BIB6) 1976; 42 Cheek, Hill (BIB7) 1974; Vol. 4 Bolander, Fellows (BIB4) 1976; 251 Gluckman (10.1016/0303-7207(84)90109-6_BIB16) 1983; 112 Rodbard (10.1016/0303-7207(84)90109-6_BIB40) 1968; 28 Wagner (10.1016/0303-7207(84)90109-6_BIB48) 1978; 47 Herington (10.1016/0303-7207(84)90109-6_BIB20) 1976; 25 Posner (10.1016/0303-7207(84)90109-6_BIB36) 1980; 107 Robertson (10.1016/0303-7207(84)90109-6_BIB38) 1981; 108 Shoer (10.1016/0303-7207(84)90109-6_BIB44) 1978; 537 Fernandez (10.1016/0303-7207(84)90109-6_BIB13) 1983; 209 Tsushima (10.1016/0303-7207(84)90109-6_BIB47) 1973; 37 Closset (10.1016/0303-7207(84)90109-6_BIB8) 1983; 214 Eshet (10.1016/0303-7207(84)90109-6_BIB11) 1982; 16 Robertson (10.1016/0303-7207(84)90109-6_BIB39) 1982; 111 Laron (10.1016/0303-7207(84)90109-6_BIB25) 1982; 18 Eakle (10.1016/0303-7207(84)90109-6_BIB10) 1982; 110 Goodman (10.1016/0303-7207(84)90109-6_BIB17) 1974; Vol. 4 Li (10.1016/0303-7207(84)90109-6_BIB27) 1945; 159 Boeynaems (10.1016/0303-7207(84)90109-6_BIB3) 1980 Shiu (10.1016/0303-7207(84)90109-6_BIB43) 1974; 140 Wallis (10.1016/0303-7207(84)90109-6_BIB49) 1978; Vol. 5 Sasaki (10.1016/0303-7207(84)90109-6_BIB41) 1982; 101 Scares (10.1016/0303-7207(84)90109-6_BIB45) 1983; 112 Herington (10.1016/0303-7207(84)90109-6_BIB21) 1983; 112 Hall (10.1016/0303-7207(84)90109-6_BIB19) 1974; 2 Surowy (10.1016/0303-7207(84)90109-6_BIB46) 1983; 2 Yudaev (10.1016/0303-7207(84)90109-6_BIB52) 1982; 16 Peel (10.1016/0303-7207(84)90109-6_BIB34) 1983; 66 Cheek (10.1016/0303-7207(84)90109-6_BIB7) 1974; Vol. 4 Evans (10.1016/0303-7207(84)90109-6_BIB12) 1931; 98 Wallis (10.1016/0303-7207(84)90109-6_BIB50) 1981; 9 Fryer (10.1016/0303-7207(84)90109-6_BIB14) 1979; 39 Posner (10.1016/0303-7207(84)90109-6_BIB35) 1976; 98 Machlin (10.1016/0303-7207(84)90109-6_BIB28) 1972; 35 Murthy (10.1016/0303-7207(84)90109-6_BIB31) 1982; 111 Delfino (10.1016/0303-7207(84)90109-6_BIB9) 1983; 21 Brumby (10.1016/0303-7207(84)90109-6_BIB5) 1959; 2 Carr (10.1016/0303-7207(84)90109-6_BIB6) 1976; 42 Pankov (10.1016/0303-7207(84)90109-6_BIB33) 1980; 45 Altszuler (10.1016/0303-7207(84)90109-6_BIB1) 1974; Vol. 4 Haeuptle (10.1016/0303-7207(84)90109-6_BIB18) 1983; 258 Nicoll (10.1016/0303-7207(84)90109-6_BIB32) 1982; 25 Hughs (10.1016/0303-7207(84)90109-6_BIB22) 1979; 105 Moore (10.1016/0303-7207(84)90109-6_BIB30) 1978; 46 Bolander (10.1016/0303-7207(84)90109-6_BIB4) 1976; 251 Waters (10.1016/0303-7207(84)90109-6_BIB51) 1979; 254 Postel-Vinay (10.1016/0303-7207(84)90109-6_BIB37) 1976; 69 Lewis (10.1016/0303-7207(84)90109-6_BIB26) 1980; 36 Kyle (10.1016/0303-7207(84)90109-6_BIB24) 1982; 169 Fukushima (10.1016/0303-7207(84)90109-6_BIB15) 1983; 21 Kostyo (10.1016/0303-7207(84)90109-6_BIB23) 1974; Vol. 4 Bauman (10.1016/0303-7207(84)90109-6_BIB2) 1982; 41 Schenkman (10.1016/0303-7207(84)90109-6_BIB42) 1978; 52 Mattera (10.1016/0303-7207(84)90109-6_BIB29) 1982; 19 |
| References_xml | – volume: 111 start-page: 2117 year: 1982 end-page: 2124 ident: BIB31 publication-title: Endocrinology contributor: fullname: Friesen – volume: Vol. 4 start-page: 187 year: 1974 end-page: 210 ident: BIB23 publication-title: Handbook of Physiology contributor: fullname: Nutting – volume: 39 start-page: 123 year: 1979 end-page: 130 ident: BIB14 publication-title: Gen. Comp. Endocrinol. contributor: fullname: Fryer – volume: 107 start-page: 1954 year: 1980 end-page: 1958 ident: BIB36 publication-title: Endocrinology contributor: fullname: Charrier – volume: 9 start-page: 33 year: 1981 end-page: 35 ident: BIB50 publication-title: Biochem. Soc. Trans. contributor: fullname: Cadman – volume: 209 start-page: 107 year: 1983 end-page: 115 ident: BIB13 publication-title: Biochem. J. contributor: fullname: Delfino – volume: 46 start-page: 374 year: 1978 end-page: 380 ident: BIB30 publication-title: J. Clin. Endocrinol. Metab. contributor: fullname: Jin – volume: Vol. 5 start-page: 213 year: 1978 end-page: 320 ident: BIB49 publication-title: Chemistry and Biochemistry of Amino Acids, Peptides and Proteins contributor: fullname: Wallis – volume: Vol. 4 start-page: 211 year: 1974 end-page: 231 ident: BIB17 publication-title: Handbook of Physiology contributor: fullname: Schwartz – volume: 41 start-page: 2538 year: 1982 end-page: 2544 ident: BIB2 publication-title: Fed. Proc. contributor: fullname: Currie – volume: 42 start-page: 484 year: 1976 end-page: 493 ident: BIB6 publication-title: J. Clin. Endocrinol. Metab. contributor: fullname: Friesen – volume: Vol. 4 start-page: 159 year: 1974 end-page: 185 ident: BIB7 publication-title: Handbook of Physiology contributor: fullname: Hill – volume: 254 start-page: 6815 year: 1979 end-page: 6825 ident: BIB51 publication-title: J. Biol. Chem. contributor: fullname: Friesen – volume: 2 start-page: 155 year: 1974 end-page: 178 ident: BIB19 publication-title: Curr. Topics Exp. Endocrinol. contributor: fullname: Van Wyk – volume: 18 start-page: 823 year: 1982 end-page: 829 ident: BIB25 publication-title: Isr. J. Med. Sci. contributor: fullname: Laron – volume: 98 start-page: 645 year: 1976 end-page: 654 ident: BIB35 publication-title: Endocrinology contributor: fullname: Posner – volume: 159 start-page: 353 year: 1945 end-page: 366 ident: BIB27 publication-title: J. Biol. Chem. contributor: fullname: Simpson – volume: Vol. 4 start-page: 233 year: 1974 end-page: 252 ident: BIB1 publication-title: Handbook of Physiology contributor: fullname: Altszuler – volume: 25 start-page: 369 year: 1982 end-page: 381 ident: BIB32 publication-title: Perspect. Biol. Med. contributor: fullname: Nicoll – volume: 2 start-page: 195 year: 1983 end-page: 208 ident: BIB46 publication-title: J. Protein Chem. contributor: fullname: Wallis – volume: 69 start-page: 137 year: 1976 end-page: 140 ident: BIB37 publication-title: FEBS Lett. contributor: fullname: Postel-Vinay – volume: 66 start-page: 776 year: 1983 end-page: 782 ident: BIB34 publication-title: J. Dairy Sci. contributor: fullname: Gorewit – volume: 16 start-page: 163 year: 1982 end-page: 175 ident: BIB52 publication-title: Endocrinol. Exp. contributor: fullname: Pankov – volume: 111 start-page: 1862 year: 1982 end-page: 1866 ident: BIB39 publication-title: Endocrinology contributor: fullname: Friesen – volume: 251 start-page: 2703 year: 1976 end-page: 2708 ident: BIB4 publication-title: J. Biol. Chem. contributor: fullname: Fellows – volume: 37 start-page: 334 year: 1973 end-page: 337 ident: BIB47 publication-title: J. Clin. Endocrinol. Metab. contributor: fullname: Friesen – volume: 258 start-page: 305 year: 1983 end-page: 314 ident: BIB18 publication-title: J. Biol. Chem. contributor: fullname: Kraehenbuhl – volume: 101 start-page: 574 year: 1982 end-page: 579 ident: BIB41 publication-title: Acta Endocrinol. (Kbh.) contributor: fullname: Matsuzaki – volume: 25 start-page: 341 year: 1976 end-page: 353 ident: BIB20 publication-title: Metabolism contributor: fullname: Daughaday – volume: 16 start-page: 901 year: 1982 ident: BIB11 publication-title: Pediatr. Res. contributor: fullname: Dintzman – volume: 98 start-page: 511 year: 1931 end-page: 546 ident: BIB12 publication-title: Am. J. Physiol. contributor: fullname: Simpson – volume: 35 start-page: 794 year: 1972 end-page: 800 ident: BIB28 publication-title: J. Anim. Sci. contributor: fullname: Machlin – volume: 45 start-page: 195 year: 1980 end-page: 209 ident: BIB33 publication-title: Biokhimiya contributor: fullname: Pozdnyakov – volume: 537 start-page: 336 year: 1978 end-page: 347 ident: BIB44 publication-title: Biochim. Biophys. Acta contributor: fullname: Talamantes – volume: 112 start-page: 1607 year: 1983 end-page: 1612 ident: BIB16 publication-title: Endocrinology contributor: fullname: Elliott – volume: 52 start-page: 83 year: 1978 end-page: 89 ident: BIB42 publication-title: Methods Enzymol. contributor: fullname: Cinti – volume: 105 start-page: 414 year: 1979 end-page: 420 ident: BIB22 publication-title: Endocrinology contributor: fullname: Hughs – volume: 19 start-page: 181 year: 1982 end-page: 186 ident: BIB29 publication-title: Int. J. Peptide Protein Res. contributor: fullname: Dellacha – volume: 112 start-page: 2032 year: 1983 end-page: 2038 ident: BIB21 publication-title: Endocrinology contributor: fullname: Graystone – volume: 169 start-page: 368 year: 1982 end-page: 375 ident: BIB24 publication-title: Proc. Soc. Exp. Biol. Med. contributor: fullname: Odell – volume: 108 start-page: 2388 year: 1981 end-page: 2390 ident: BIB38 publication-title: Endocrinology contributor: fullname: Friesen – volume: 36 start-page: 477 year: 1980 end-page: 508 ident: BIB26 publication-title: Recent Progr. Hormone Res. contributor: fullname: Vanderlaan – volume: 110 start-page: 1758 year: 1982 end-page: 1765 ident: BIB10 publication-title: Endocrinology contributor: fullname: Bremel – volume: 47 start-page: 397 year: 1978 ident: BIB48 publication-title: J. Anim. Sci. contributor: fullname: Veenhuizen – volume: 28 start-page: 1412 year: 1968 end-page: 1418 ident: BIB40 publication-title: J. Clin. Endocrinol. Metab. contributor: fullname: Ross – volume: 21 start-page: 440 year: 1983 end-page: 450 ident: BIB9 publication-title: Int. J. Peptide Protein Res. contributor: fullname: Santome – volume: 112 start-page: 1313 year: 1983 end-page: 1317 ident: BIB45 publication-title: Endocrinology contributor: fullname: Talamantes – volume: 140 start-page: 301 year: 1974 end-page: 311 ident: BIB43 publication-title: Biochem. J. contributor: fullname: Friesen – volume: 21 start-page: 451 year: 1983 end-page: 457 ident: BIB15 publication-title: Int. J. Peptide Protein Res. contributor: fullname: Santomé – volume: 2 start-page: 683 year: 1959 end-page: 689 ident: BIB5 publication-title: N.Z. J. Agric. Res. contributor: fullname: Brumby – volume: 214 start-page: 885 year: 1983 end-page: 892 ident: BIB8 publication-title: Biochem. J. contributor: fullname: Hennen – start-page: 18 year: 1980 ident: BIB3 article-title: Outlines of Receptor Theory contributor: fullname: Dumont – volume: 140 start-page: 301 year: 1974 ident: 10.1016/0303-7207(84)90109-6_BIB43 publication-title: Biochem. J. doi: 10.1042/bj1400301 contributor: fullname: Shiu – volume: 47 start-page: 397 year: 1978 ident: 10.1016/0303-7207(84)90109-6_BIB48 publication-title: J. Anim. Sci. contributor: fullname: Wagner – volume: 52 start-page: 83 year: 1978 ident: 10.1016/0303-7207(84)90109-6_BIB42 publication-title: Methods Enzymol. doi: 10.1016/S0076-6879(78)52008-9 contributor: fullname: Schenkman – volume: 258 start-page: 305 year: 1983 ident: 10.1016/0303-7207(84)90109-6_BIB18 publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(18)33257-5 contributor: fullname: Haeuptle – volume: 2 start-page: 155 year: 1974 ident: 10.1016/0303-7207(84)90109-6_BIB19 publication-title: Curr. Topics Exp. Endocrinol. doi: 10.1016/B978-0-12-153202-4.50012-1 contributor: fullname: Hall – volume: 21 start-page: 451 year: 1983 ident: 10.1016/0303-7207(84)90109-6_BIB15 publication-title: Int. J. Peptide Protein Res. doi: 10.1111/j.1399-3011.1983.tb03126.x contributor: fullname: Fukushima – volume: 37 start-page: 334 year: 1973 ident: 10.1016/0303-7207(84)90109-6_BIB47 publication-title: J. Clin. Endocrinol. Metab. doi: 10.1210/jcem-37-2-334 contributor: fullname: Tsushima – volume: 16 start-page: 901 year: 1982 ident: 10.1016/0303-7207(84)90109-6_BIB11 publication-title: Pediatr. Res. contributor: fullname: Eshet – volume: 28 start-page: 1412 year: 1968 ident: 10.1016/0303-7207(84)90109-6_BIB40 publication-title: J. Clin. Endocrinol. Metab. doi: 10.1210/jcem-28-10-1412 contributor: fullname: Rodbard – volume: 112 start-page: 1607 year: 1983 ident: 10.1016/0303-7207(84)90109-6_BIB16 publication-title: Endocrinology doi: 10.1210/endo-112-5-1607 contributor: fullname: Gluckman – volume: Vol. 4 start-page: 187 year: 1974 ident: 10.1016/0303-7207(84)90109-6_BIB23 contributor: fullname: Kostyo – volume: 159 start-page: 353 year: 1945 ident: 10.1016/0303-7207(84)90109-6_BIB27 publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(19)52796-X contributor: fullname: Li – volume: Vol. 4 start-page: 233 year: 1974 ident: 10.1016/0303-7207(84)90109-6_BIB1 contributor: fullname: Altszuler – volume: 45 start-page: 195 year: 1980 ident: 10.1016/0303-7207(84)90109-6_BIB33 publication-title: Biokhimiya contributor: fullname: Pankov – volume: 46 start-page: 374 year: 1978 ident: 10.1016/0303-7207(84)90109-6_BIB30 publication-title: J. Clin. Endocrinol. Metab. doi: 10.1210/jcem-46-3-374 contributor: fullname: Moore – volume: 112 start-page: 2032 year: 1983 ident: 10.1016/0303-7207(84)90109-6_BIB21 publication-title: Endocrinology doi: 10.1210/endo-112-6-2032 contributor: fullname: Herington – volume: 110 start-page: 1758 year: 1982 ident: 10.1016/0303-7207(84)90109-6_BIB10 publication-title: Endocrinology doi: 10.1210/endo-110-5-1758 contributor: fullname: Eakle – start-page: 18 year: 1980 ident: 10.1016/0303-7207(84)90109-6_BIB3 article-title: Outlines of Receptor Theory contributor: fullname: Boeynaems – volume: 98 start-page: 511 year: 1931 ident: 10.1016/0303-7207(84)90109-6_BIB12 publication-title: Am. J. Physiol. doi: 10.1152/ajplegacy.1931.98.3.511 contributor: fullname: Evans – volume: 105 start-page: 414 year: 1979 ident: 10.1016/0303-7207(84)90109-6_BIB22 publication-title: Endocrinology doi: 10.1210/endo-105-2-414 contributor: fullname: Hughs – volume: 42 start-page: 484 year: 1976 ident: 10.1016/0303-7207(84)90109-6_BIB6 publication-title: J. Clin. Endocrinol. Metab. doi: 10.1210/jcem-42-3-484 contributor: fullname: Carr – volume: 21 start-page: 440 year: 1983 ident: 10.1016/0303-7207(84)90109-6_BIB9 publication-title: Int. J. Peptide Protein Res. doi: 10.1111/j.1399-3011.1983.tb03125.x contributor: fullname: Delfino – volume: 111 start-page: 1862 year: 1982 ident: 10.1016/0303-7207(84)90109-6_BIB39 publication-title: Endocrinology doi: 10.1210/endo-111-6-1862 contributor: fullname: Robertson – volume: 101 start-page: 574 year: 1982 ident: 10.1016/0303-7207(84)90109-6_BIB41 publication-title: Acta Endocrinol. (Kbh.) doi: 10.1530/acta.0.1010574 contributor: fullname: Sasaki – volume: 36 start-page: 477 year: 1980 ident: 10.1016/0303-7207(84)90109-6_BIB26 publication-title: Recent Progr. Hormone Res. contributor: fullname: Lewis – volume: 39 start-page: 123 year: 1979 ident: 10.1016/0303-7207(84)90109-6_BIB14 publication-title: Gen. Comp. Endocrinol. doi: 10.1016/0016-6480(79)90217-X contributor: fullname: Fryer – volume: 209 start-page: 107 year: 1983 ident: 10.1016/0303-7207(84)90109-6_BIB13 publication-title: Biochem. J. doi: 10.1042/bj2090107 contributor: fullname: Fernandez – volume: 18 start-page: 823 year: 1982 ident: 10.1016/0303-7207(84)90109-6_BIB25 publication-title: Isr. J. Med. Sci. contributor: fullname: Laron – volume: 98 start-page: 645 year: 1976 ident: 10.1016/0303-7207(84)90109-6_BIB35 publication-title: Endocrinology doi: 10.1210/endo-98-3-645 contributor: fullname: Posner – volume: 108 start-page: 2388 year: 1981 ident: 10.1016/0303-7207(84)90109-6_BIB38 publication-title: Endocrinology doi: 10.1210/endo-108-6-2388 contributor: fullname: Robertson – volume: 2 start-page: 683 year: 1959 ident: 10.1016/0303-7207(84)90109-6_BIB5 publication-title: N.Z. J. Agric. Res. doi: 10.1080/00288233.1959.10422827 contributor: fullname: Brumby – volume: 16 start-page: 163 year: 1982 ident: 10.1016/0303-7207(84)90109-6_BIB52 publication-title: Endocrinol. Exp. doi: 10.1111/j.1365-2265.1982.tb03160.x contributor: fullname: Yudaev – volume: 25 start-page: 341 year: 1976 ident: 10.1016/0303-7207(84)90109-6_BIB20 publication-title: Metabolism doi: 10.1016/0026-0495(76)90092-5 contributor: fullname: Herington – volume: 537 start-page: 336 year: 1978 ident: 10.1016/0303-7207(84)90109-6_BIB44 publication-title: Biochim. Biophys. Acta doi: 10.1016/0005-2795(78)90517-2 contributor: fullname: Shoer – volume: 2 start-page: 195 year: 1983 ident: 10.1016/0303-7207(84)90109-6_BIB46 publication-title: J. Protein Chem. doi: 10.1007/BF01025354 contributor: fullname: Surowy – volume: 25 start-page: 369 year: 1982 ident: 10.1016/0303-7207(84)90109-6_BIB32 publication-title: Perspect. Biol. Med. doi: 10.1353/pbm.1982.0025 contributor: fullname: Nicoll – volume: 107 start-page: 1954 year: 1980 ident: 10.1016/0303-7207(84)90109-6_BIB36 publication-title: Endocrinology doi: 10.1210/endo-107-6-1954 contributor: fullname: Posner – volume: 35 start-page: 794 year: 1972 ident: 10.1016/0303-7207(84)90109-6_BIB28 publication-title: J. Anim. Sci. doi: 10.2527/jas1972.354794x contributor: fullname: Machlin – volume: 41 start-page: 2538 year: 1982 ident: 10.1016/0303-7207(84)90109-6_BIB2 contributor: fullname: Bauman – volume: 19 start-page: 181 year: 1982 ident: 10.1016/0303-7207(84)90109-6_BIB29 publication-title: Int. J. Peptide Protein Res. doi: 10.1111/j.1399-3011.1982.tb02607.x contributor: fullname: Mattera – volume: Vol. 5 start-page: 213 year: 1978 ident: 10.1016/0303-7207(84)90109-6_BIB49 contributor: fullname: Wallis – volume: Vol. 4 start-page: 159 year: 1974 ident: 10.1016/0303-7207(84)90109-6_BIB7 contributor: fullname: Cheek – volume: 69 start-page: 137 year: 1976 ident: 10.1016/0303-7207(84)90109-6_BIB37 publication-title: FEBS Lett. doi: 10.1016/0014-5793(76)80670-9 contributor: fullname: Postel-Vinay – volume: Vol. 4 start-page: 211 year: 1974 ident: 10.1016/0303-7207(84)90109-6_BIB17 contributor: fullname: Goodman – volume: 214 start-page: 885 year: 1983 ident: 10.1016/0303-7207(84)90109-6_BIB8 publication-title: Biochem. J. doi: 10.1042/bj2140885 contributor: fullname: Closset – volume: 169 start-page: 368 year: 1982 ident: 10.1016/0303-7207(84)90109-6_BIB24 contributor: fullname: Kyle – volume: 9 start-page: 33 year: 1981 ident: 10.1016/0303-7207(84)90109-6_BIB50 publication-title: Biochem. Soc. Trans. doi: 10.1042/bst0090033 contributor: fullname: Wallis – volume: 111 start-page: 2117 year: 1982 ident: 10.1016/0303-7207(84)90109-6_BIB31 publication-title: Endocrinology doi: 10.1210/endo-111-6-2117 contributor: fullname: Murthy – volume: 254 start-page: 6815 year: 1979 ident: 10.1016/0303-7207(84)90109-6_BIB51 publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(18)50441-5 contributor: fullname: Waters – volume: 251 start-page: 2703 year: 1976 ident: 10.1016/0303-7207(84)90109-6_BIB4 publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(17)33544-5 contributor: fullname: Bolander – volume: 66 start-page: 776 year: 1983 ident: 10.1016/0303-7207(84)90109-6_BIB34 publication-title: J. Dairy Sci. doi: 10.3168/jds.S0022-0302(83)81857-8 contributor: fullname: Peel – volume: 112 start-page: 1313 year: 1983 ident: 10.1016/0303-7207(84)90109-6_BIB45 publication-title: Endocrinology doi: 10.1210/endo-112-4-1313 contributor: fullname: Scares |
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| Snippet | A homologous radioreceptor assay using recombinant bovine growth hormone and bovine liver membranes is described. The total specific binding of
125I-labeled... A homologous radioreceptor assay using recombinant bovine growth hormone and bovine liver membranes is described. The total specific binding of 125I-labeled... A homologous radioreceptor assay using recombinant bovine growth hormone and bovine liver membranes is described. The total specific binding of... |
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| SubjectTerms | Animals assays Biological and medical sciences bovine growth hormone Cattle Cell physiology Fundamental and applied biological sciences. Psychology Growth Hormone growth hormone receptor homologous hormone-receptor system Hormonal regulation Hydrogen-Ion Concentration In Vitro Techniques Iodine Radioisotopes Kinetics laboratory animals Membranes - metabolism Microsomes, Liver - metabolism Molecular and cellular biology Placental Lactogen - pharmacology Prolactin - pharmacology Proteins - metabolism Radioligand Assay - methods radioreceptor assay Receptors, Cell Surface - metabolism Receptors, Somatotropin somatogenic Temperature Time Factors |
| Title | Homologous somatotropin radioreceptor assay utilizing recombinant bovine growth hormone |
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