PCR based cloning and sequencing of isogenes encoding the tree pollen major allergen Car b I from Carpinus betulus, hornbeam

• Published in: Molecular immunology Vol. 29; no. 6; p. 703
• Main Authors: Larsen, J N, Strøman, P, Ipsen, H
• Format: Journal Article
• Language: English
• Published: England 01.06.1992
• Subjects: Allergens - genetics; Allergens - immunology; Amino Acid Sequence; Antigens, Plant; Base Sequence; Blotting, Western; Cloning, Molecular; Genes, Plant; Immunoelectrophoresis, Two-Dimensional; Molecular Sequence Data; Oligodeoxyribonucleotides - chemistry; Plant Proteins - genetics; Plant Proteins - immunology; Pollen - genetics; Polymerase Chain Reaction; Polymorphism, Genetic; Recombinant Proteins - immunology; Sequence Alignment; Solubility; Trees - immunology

Cloning of the gene encoding the major allergen, Car b I, from Carpinus betulus (hornbeam) pollen was performed using the Polymerase Chain Reaction (PCR) to specifically amplify the gene of interest using single stranded cDNA as template. Specific primers, deduced from the aminoterminal sequence of...