Differential Phosphorylation of the Gap Junction Protein Connexin43 in Junctional Communication-Competent and -Deficient Cell Lines
Connexin43 is a member of the highly homologous connexin family of gap junction proteins. We have studied how connexin monomers are assembled into functional gap junction plaques by examining the biosynthesis of connexin43 in cell types that differ greatly in their ability to form functional gap jun...
Saved in:
Published in | The Journal of cell biology Vol. 111; no. 5; pp. 2077 - 2088 |
---|---|
Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
New York, NY
Rockefeller University Press
01.11.1990
The Rockefeller University Press |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Connexin43 is a member of the highly homologous connexin family of gap junction proteins. We have studied how connexin monomers are assembled into functional gap junction plaques by examining the biosynthesis of connexin43 in cell types that differ greatly in their ability to form functional gap junctions. Using a combination of metabolic radiolabeling and immunoprecipitation, we have shown that connexin43 is synthesized in gap junctional communication-competent cells as a 42-kD protein that is efficiently converted to a ∼46-kD species (connexin43- P2) by the posttranslational addition of phosphate. Surprisingly, certain cell lines severely deficient in gap junctional communication and known cell-cell adhesion molecules (S180 and L929 cells) also expressed 42-kD connexin43. Connexin43 in these communication-deficient cell lines was not, however, phosphorylated to the P2 form. Conversion of S180 cells to a communication-competent phenotype by transfection with a cDNA encoding the cell-cell adhesion molecule L-CAM induced phosphorylation of connexin43 to the P2 form; conversely, blocking junctional communication in ordinarily communication-competent cells inhibited connexin43- P2 formation. Immunohistochemical localization studies indicated that only communication-competent cells accumulated connexin43 in visible gap junction plaques. Together, these results establish a strong correlation between the ability of cells to process connexin43 to the P2 form and to produce functional gap junctions. Connexin43 phosphorylation may therefore play a functional role in gap junction assembly and/or activity. |
---|---|
AbstractList | Connexin43 is a member of the highly homologous connexin family of gap junction proteins. We have studied how connexin monomers are assembled into functional gap junction plaques by examining the biosynthesis of connexin43 in cell types that differ greatly in their ability to form functional gap junctions. Using a combination of metabolic radiolabeling and immunoprecipitation, we have shown that connexin43 is synthesized in gap junctional communication-competent cells as a 42-kD protein that is efficiently converted to a approximately 46-kD species (connexin43-P2) by the posttranslational addition of phosphate. Surprisingly, certain cell lines severely deficient in gap junctional communication and known cell-cell adhesion molecules (S180 and L929 cells) also expressed 42-kD connexin43. Connexin43 in these communication-deficient cell lines was not, however, phosphorylated to the P2 form. Conversion of S180 cells to a communication-competent phenotype by transfection with a cDNA encoding the cell-cell adhesion molecule L-CAM induced phosphorylation of connexin43 to the P2 form; conversely, blocking junctional communication in ordinarily communication-competent cells inhibited connexin43-P2 formation. Immunohistochemical localization studies indicated that only communication-competent cells accumulated connexin43 in visible gap junction plaques. Together, these results establish a strong correlation between the ability of cells to process connexin43 to the P2 form and to produce functional gap junctions. Connexin43 phosphorylation may therefore play a functional role in gap junction assembly and/or activity. Connexin43 is a member of the highly homologous connexin family of gap junction proteins. We have studied how connexin monomers are assembled into functional gap junction plaques by examining the biosynthesis of connexin43 in cell types that differ greatly in their ability to form functional gap junctions. Using a combination of metabolic radiolabeling and immunoprecipitation, we have shown that connexin43 is synthesized in gap junctional communication-competent cells as a 42-kD protein that is efficiently converted to a ∼46-kD species (connexin43- P2) by the posttranslational addition of phosphate. Surprisingly, certain cell lines severely deficient in gap junctional communication and known cell-cell adhesion molecules (S180 and L929 cells) also expressed 42-kD connexin43. Connexin43 in these communication-deficient cell lines was not, however, phosphorylated to the P2 form. Conversion of S180 cells to a communication-competent phenotype by transfection with a cDNA encoding the cell-cell adhesion molecule L-CAM induced phosphorylation of connexin43 to the P2 form; conversely, blocking junctional communication in ordinarily communication-competent cells inhibited connexin43- P2 formation. Immunohistochemical localization studies indicated that only communication-competent cells accumulated connexin43 in visible gap junction plaques. Together, these results establish a strong correlation between the ability of cells to process connexin43 to the P2 form and to produce functional gap junctions. Connexin43 phosphorylation may therefore play a functional role in gap junction assembly and/or activity. |
Author | Edelman, Gerald M. Cunningham, Bruce A. Goodenough, Daniel A. Musil, Linda S. |
Author_xml | – sequence: 1 givenname: Linda S. surname: Musil fullname: Musil, Linda S. – sequence: 2 givenname: Bruce A. surname: Cunningham fullname: Cunningham, Bruce A. – sequence: 3 givenname: Gerald M. surname: Edelman fullname: Edelman, Gerald M. – sequence: 4 givenname: Daniel A. surname: Goodenough fullname: Goodenough, Daniel A. |
BackLink | http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19421380$$DView record in Pascal Francis https://www.ncbi.nlm.nih.gov/pubmed/2172261$$D View this record in MEDLINE/PubMed |
BookMark | eNpVkcFvFCEYxYmpqdvVqydNuNTbTPlgZmAuJmZqq2aT9qBnwrLgspmBEWZMe_Yfl-1uWz2Rx3v8-OCdoRMfvEHoLZASiGAXO70uAaCsS0o4f4EWUFekEFCRE7QghELR1rR-hc5S2hFCKl6xU3RKgVPawAL9uXTWmmj85FSPb7chjdsQ73s1ueBxsHjaGnytRvxt9vph7zaGyTiPu-C9uXO-YjirRztDujAMs3f6AVFkNZop87HyG1xcGuu028vO9D1eOW_Sa_TSqj6ZN8d1iX5cff7efSlWN9dfu0-rQlfAedG0SrFmY2vB1loTIQwXlDeC1O2GkpzQCmwj9BqMBS4qrVtuuchxk1ONZkv08cAd5_VgNjpPEVUvx-gGFe9lUE7-73i3lT_Db0kBGsZoBnw4AmL4NZs0ycElnd-hvAlzkoIQwev8w0tUHoI6hpSisU-XAJH72mSuTebaZC33teUD7_8d7Sl-7Cn750dfJa16G5XXLj1T24oCEyTn3h1yuzSF-Ow3UNFGsL_Y4K2- |
CODEN | JCLBA3 |
CitedBy_id | crossref_primary_10_1006_jmbi_2001_5262 crossref_primary_10_1242_jcs_114_22_4013 crossref_primary_10_1242_jcs_110_3_301 crossref_primary_10_1016_j_bbamem_2005_11_004 crossref_primary_10_1109_LPT_2021_3049348 crossref_primary_10_1007_BF00374154 crossref_primary_10_1091_mbc_e05_05_0415 crossref_primary_10_1074_jbc_M005826200 crossref_primary_10_1002_jbm_a_30753 crossref_primary_10_3389_fphar_2018_00814 crossref_primary_10_1111_j_1432_1033_1995_670_b_x crossref_primary_10_1002_mc_2940110305 crossref_primary_10_1002_jemt_1070310507 crossref_primary_10_1002_jcb_21018 crossref_primary_10_1016_j_bbamem_2004_01_007 crossref_primary_10_1093_jn_135_11_2507 crossref_primary_10_1016_j_ydbio_2004_04_031 crossref_primary_10_1242_jcs_111_6_833 crossref_primary_10_1002_jcp_1041530124 crossref_primary_10_1016_j_neulet_2006_06_025 crossref_primary_10_1002__SICI_1520_6408_1997_20_2_75__AID_DVG1_3_0_CO_2_5 crossref_primary_10_1007_s00204_004_0638_0 crossref_primary_10_1016_j_bbamem_2004_09_013 crossref_primary_10_1073_pnas_022257499 crossref_primary_10_1007_s00441_014_2020_8 crossref_primary_10_1007_s00210_012_0795_2 crossref_primary_10_1111_j_1471_4159_2008_05405_x crossref_primary_10_1017_S0967199400001970 crossref_primary_10_1046_j_1460_9568_2000_00162_x crossref_primary_10_1091_mbc_e05_08_0737 crossref_primary_10_1080_15419060490951781 crossref_primary_10_1093_molehr_7_7_671 crossref_primary_10_1046_j_0953_816x_2001_01868_x crossref_primary_10_1083_jcb_200111045 crossref_primary_10_1080_15419060600631839 crossref_primary_10_1002_jnr_20355 crossref_primary_10_1080_15419060601077917 crossref_primary_10_1016_S0378_5955_00_00047_2 crossref_primary_10_1016_S0014_4827_03_00124_1 crossref_primary_10_1074_jbc_M209427200 crossref_primary_10_1242_jcs_01056 crossref_primary_10_1016_j_bbamem_2004_09_009 crossref_primary_10_1523_JNEUROSCI_18_07_02520_1998 crossref_primary_10_1002_1097_0029_20010201_52_3_331__AID_JEMT1017_3_0_CO_2_H crossref_primary_10_1007_BF02110112 crossref_primary_10_1016_0892_0354_92_90014_H crossref_primary_10_1016_j_yjmcc_2009_05_004 crossref_primary_10_1016_S0009_2797_97_03759_9 crossref_primary_10_1242_jcs_110_3_311 crossref_primary_10_1007_BF02110107 crossref_primary_10_1002__SICI_1098_2744_199603_15_3_190__AID_MC5_3_0_CO_2_M crossref_primary_10_1074_jbc_M213283200 crossref_primary_10_1016_S1065_6995_02_00281_0 crossref_primary_10_1002_jcp_21412 crossref_primary_10_1016_j_actbio_2012_03_039 crossref_primary_10_1016_S1043_2760_02_00623_9 crossref_primary_10_1016_0006_8993_94_90242_9 crossref_primary_10_1177_00220345970760070501 crossref_primary_10_1083_jcb_143_6_1735 crossref_primary_10_3109_02713689609007625 crossref_primary_10_1074_jbc_270_44_26581 crossref_primary_10_1006_dbio_1998_8951 crossref_primary_10_1097_MJT_0b013e31818be50f crossref_primary_10_1152_ajpheart_00285_2006 crossref_primary_10_1006_bbrc_2001_4710 crossref_primary_10_1006_dbio_1997_8703 crossref_primary_10_1242_jcs_115_16_3241 crossref_primary_10_1006_exer_2000_0847 crossref_primary_10_1083_jcb_137_4_847 crossref_primary_10_1002_ijc_26392 crossref_primary_10_1016_0165_3806_92_90077_A crossref_primary_10_1002_jcp_1041580306 crossref_primary_10_1002_syn_890130207 crossref_primary_10_1006_taap_1998_8516 crossref_primary_10_1016_S1043_4682_10_80007_8 crossref_primary_10_1529_biophysj_103_036350 crossref_primary_10_1002_pros_20451 crossref_primary_10_1002_glia_20471 crossref_primary_10_1242_jcs_114_4_763 crossref_primary_10_1111_j_1460_9568_2006_05185_x crossref_primary_10_1016_j_yjmcc_2005_12_010 crossref_primary_10_1074_jbc_M709288200 crossref_primary_10_1021_jf103205c crossref_primary_10_1002__SICI_1098_2744_199810_23_2_121__AID_MC9_3_0_CO_2_P crossref_primary_10_1002_mc_10160 crossref_primary_10_1590_S0100_879X1997000500003 crossref_primary_10_1002__SICI_1098_2795_200004_55_4_393__AID_MRD6_3_0_CO_2_X crossref_primary_10_1016_S1043_4682_10_80008_X crossref_primary_10_1074_jbc_275_33_25207 crossref_primary_10_1073_pnas_96_20_11613 crossref_primary_10_1080_15419060500511875 crossref_primary_10_1091_mbc_11_6_1933 crossref_primary_10_1080_12265071_1998_9647396 crossref_primary_10_1242_jcs_1993_Supplement_17_19 crossref_primary_10_1002_aja_1001940403 crossref_primary_10_1002_dneu_20652 crossref_primary_10_1074_jbc_273_3_1519 crossref_primary_10_1016_S0005_2736_02_00504_7 crossref_primary_10_1002_ijc_2910560116 crossref_primary_10_1016_S0022_5347_17_36455_8 crossref_primary_10_1046_j_1460_9568_2000_01331_x crossref_primary_10_1016_j_bone_2008_01_025 crossref_primary_10_1091_mbc_e04_03_0271 crossref_primary_10_1111_1523_1747_ep12393218 crossref_primary_10_1016_S0304_3835_98_00082_2 crossref_primary_10_1002_jbm_10239 crossref_primary_10_1002_dvdy_20426 crossref_primary_10_1016_j_bbamem_2011_09_025 crossref_primary_10_1074_jbc_M110_217208 crossref_primary_10_1016_j_bbamem_2017_05_008 crossref_primary_10_1074_jbc_M901619200 crossref_primary_10_1159_000110459 crossref_primary_10_1242_jcs_1993_Supplement_17_22 crossref_primary_10_1111_j_1432_1033_1996_0001q_x crossref_primary_10_1002_ijc_2910540518 crossref_primary_10_1111_j_1432_0436_1992_tb00675_x crossref_primary_10_1016_S0306_4522_99_00411_X crossref_primary_10_1242_jcs_108_11_3501 crossref_primary_10_1242_jcs_03149 crossref_primary_10_3390_biom13061005 crossref_primary_10_1186_1471_2091_11_14 crossref_primary_10_1242_jcs_107_5_1369 crossref_primary_10_1002__SICI_1521_4141_199906_29_06_1793__AID_IMMU1793_3_0_CO_2_3 crossref_primary_10_1074_jbc_272_36_22824 crossref_primary_10_1093_carcin_22_2_221 crossref_primary_10_1016_S1043_4682_10_80005_4 crossref_primary_10_1016_S0304_3835_98_00060_3 crossref_primary_10_3390_cells13060495 crossref_primary_10_1083_jcb_200101057 crossref_primary_10_3109_15419069409014208 crossref_primary_10_1091_mbc_e09_01_0062 crossref_primary_10_1016_S0146_0005_95_80046_8 crossref_primary_10_1021_jf051747h crossref_primary_10_1152_ajpcell_2001_281_4_C1211 crossref_primary_10_1359_jbmr_2000_15_5_919 crossref_primary_10_1002__SICI_1097_4652_199904_179_1_87__AID_JCP11_3_0_CO_2_K crossref_primary_10_1006_exer_1999_0670 crossref_primary_10_1002__SICI_1098_2744_199812_23_4_254__AID_MC9_3_0_CO_2_4 crossref_primary_10_1017_S1431927600026477 crossref_primary_10_1006_jmcc_2000_1308 crossref_primary_10_1016_0014_4827_92_90388_O crossref_primary_10_1074_jbc_M109640200 crossref_primary_10_1016_j_bbamem_2011_07_028 crossref_primary_10_1016_S0959_440X_98_80131_0 crossref_primary_10_1161_CIRCRESAHA_113_301055 crossref_primary_10_1359_jbmr_2000_15_6_1024 crossref_primary_10_1016_0167_4889_91_90016_Q crossref_primary_10_1152_physrev_00007_2003 crossref_primary_10_1091_mbc_e04_04_0324 crossref_primary_10_1242_jcs_111_23_3529 crossref_primary_10_1006_excr_2001_5357 crossref_primary_10_3171_jns_1996_84_5_0839 crossref_primary_10_1083_jcb_153_5_1049 crossref_primary_10_1006_excr_1999_4574 crossref_primary_10_1016_S1043_4682_10_80006_6 crossref_primary_10_1002_ijc_2910510404 crossref_primary_10_1523_JNEUROSCI_18_19_07881_1998 crossref_primary_10_1002_jnr_490360403 crossref_primary_10_1016_S0002_9440_10_64438_0 crossref_primary_10_1083_jcb_137_1_203 crossref_primary_10_1111_j_1600_0854_2011_01220_x crossref_primary_10_1083_jcb_149_7_1513 crossref_primary_10_1016_S1382_6689_97_00021_5 crossref_primary_10_1002_mrd_10075 crossref_primary_10_1242_jcs_107_1_83 crossref_primary_10_1093_carcin_21_6_1097 crossref_primary_10_1152_physiol_00038_2012 crossref_primary_10_1242_jcs_108_9_3079 crossref_primary_10_1074_jbc_M304785200 crossref_primary_10_1177_0022034513489992 crossref_primary_10_3390_biom13121796 crossref_primary_10_1016_j_canlet_2011_05_016 crossref_primary_10_1089_ard_1992_2_153 crossref_primary_10_1078_0171_9335_00080 crossref_primary_10_1038_jcbfm_2010_41 crossref_primary_10_1016_S0021_9258_18_43842_2 crossref_primary_10_1523_JNEUROSCI_16_14_04311_1996 crossref_primary_10_1359_jbmr_1997_12_8_1165 crossref_primary_10_1007_BF00386175 crossref_primary_10_1016_j_bbamem_2004_10_001 crossref_primary_10_1074_jbc_271_7_3779 crossref_primary_10_3109_15419069409004449 crossref_primary_10_1016_S0197_0186_02_00016_5 crossref_primary_10_1242_jcs_108_9_3091 crossref_primary_10_1006_excr_1999_4485 crossref_primary_10_1002_jnr_23726 crossref_primary_10_3109_02713689209000061 crossref_primary_10_3390_ijms22094413 crossref_primary_10_1002__SICI_1097_4652_200007_184_1_110__AID_JCP12_3_0_CO_2_8 crossref_primary_10_1002_jcp_24982 crossref_primary_10_1007_s00232_007_9054_8 crossref_primary_10_1359_jbmr_1998_13_2_218 crossref_primary_10_1354_vp_42_5_633 crossref_primary_10_1111_j_1600_079X_1993_tb00487_x crossref_primary_10_1002_tcm_1770140603 crossref_primary_10_1254_jphs_08327FP crossref_primary_10_1248_bpb_34_1390 crossref_primary_10_1152_ajpheart_00684_2006 crossref_primary_10_1002_hep_510290443 crossref_primary_10_1016_S0304_3835_02_00620_1 crossref_primary_10_1210_en_2007_0767 crossref_primary_10_1093_eurheartj_ehaa821 crossref_primary_10_1080_1028602021000049069 crossref_primary_10_1007_s00210_008_0317_4 crossref_primary_10_1016_S0021_9258_19_50044_8 crossref_primary_10_1007_BF01994355 crossref_primary_10_1242_jcs_108_7_2609 crossref_primary_10_1046_j_1462_5822_2002_00197_x crossref_primary_10_1007_BF02948353 crossref_primary_10_1007_BF02484402 crossref_primary_10_1021_pr300790h crossref_primary_10_1590_S0100_879X1998000500001 crossref_primary_10_1002_jmor_1052220207 crossref_primary_10_3109_15419069809005602 crossref_primary_10_1007_s00232_012_9500_0 crossref_primary_10_1007_BF01076779 crossref_primary_10_1002_cbf_290130112 crossref_primary_10_1369_jhc_4A6611_2005 crossref_primary_10_1007_s00125_011_2409_9 crossref_primary_10_1074_jbc_M112_382572 crossref_primary_10_1091_mbc_e06_04_0280 crossref_primary_10_1007_s10565_011_9182_x crossref_primary_10_1371_journal_pone_0106437 crossref_primary_10_1002_mc_2940080406 crossref_primary_10_1074_jbc_M113_508390 crossref_primary_10_1016_j_bbrc_2008_06_095 crossref_primary_10_1080_15419060802014370 crossref_primary_10_1016_j_bbamcr_2012_10_020 crossref_primary_10_1083_jcb_137_5_1103 crossref_primary_10_1074_jbc_M412921200 crossref_primary_10_1007_s11010_010_0548_9 crossref_primary_10_1095_biolreprod66_3_568 crossref_primary_10_1074_jbc_M106073200 crossref_primary_10_1080_cac_10_4_6_329_333 crossref_primary_10_1111_j_1600_0773_1996_tb00236_x crossref_primary_10_1002_ar_1092410412 crossref_primary_10_1016_j_neuroscience_2004_03_031 crossref_primary_10_1016_j_bbamem_2005_07_010 crossref_primary_10_1016_1044_5765_91_90044_O crossref_primary_10_1152_ajpregu_00044_2020 crossref_primary_10_1016_S1357_2725_03_00264_4 crossref_primary_10_1080_15287390252808109 crossref_primary_10_1006_excr_1997_3716 crossref_primary_10_1002_jbm_a_30090 crossref_primary_10_1006_excr_2002_5574 crossref_primary_10_1083_jcb_146_1_219 crossref_primary_10_1006_dbio_1998_9030 crossref_primary_10_1006_abbi_2000_2131 crossref_primary_10_1167_iovs_62_14_27 crossref_primary_10_1016_S0027_5107_96_00203_5 crossref_primary_10_1007_BF01969270 crossref_primary_10_1046_j_1365_201X_2003_01196_x crossref_primary_10_1054_tice_2000_0096 crossref_primary_10_1152_ajplung_1999_276_5_L825 crossref_primary_10_1111_j_1440_1681_1996_tb01170_x crossref_primary_10_1038_srep29667 crossref_primary_10_1080_10286029808039842 crossref_primary_10_1006_excr_1997_3628 crossref_primary_10_3390_ijms14022242 crossref_primary_10_1002__SICI_1097_0215_19971009_73_2_240__AID_IJC14_3_0_CO_2_J crossref_primary_10_1083_jcb_139_7_1785 crossref_primary_10_1002_path_1140 crossref_primary_10_1016_j_hrthm_2004_02_003 crossref_primary_10_1007_s00429_017_1448_5 crossref_primary_10_1262_jrd_19082 crossref_primary_10_1152_ajpregu_00500_2010 crossref_primary_10_1091_mbc_e11_05_0438 crossref_primary_10_1074_jbc_270_21_12751 crossref_primary_10_1097_00006676_200211000_00009 crossref_primary_10_1007_BF00713370 crossref_primary_10_1186_1465_9921_7_122 crossref_primary_10_1002_aja_1002000102 crossref_primary_10_3109_15419069809040283 crossref_primary_10_1002__SICI_1520_6408_1999_24_1_2_137__AID_DVG13_3_0_CO_2_7 crossref_primary_10_1242_dev_116_Supplement_99 crossref_primary_10_1089_ars_2008_2119 crossref_primary_10_1152_ajplung_2001_280_6_L1085 crossref_primary_10_1016_j_biomaterials_2007_07_027 crossref_primary_10_1002_eji_1830250219 crossref_primary_10_1152_ajplung_2001_280_4_L680 crossref_primary_10_1161_CIRCRESAHA_111_247023 crossref_primary_10_1093_carcin_21_5_097 crossref_primary_10_3390_ijms21155216 crossref_primary_10_1002__SICI_1098_2744_199806_22_2_120__AID_MC7_3_0_CO_2_Q crossref_primary_10_1242_dev_121_2_371 crossref_primary_10_1074_jbc_274_15_10489 crossref_primary_10_1042_BJ20082319 crossref_primary_10_1016_j_cellsig_2006_11_007 crossref_primary_10_1046_j_1471_4159_1994_62062394_x crossref_primary_10_1242_jcs_113_9_1535 crossref_primary_10_1006_exnr_1999_7027 crossref_primary_10_3390_ijms222111599 crossref_primary_10_1002_jcb_10610 crossref_primary_10_1083_jcb_137_6_1393 crossref_primary_10_1074_jbc_M109_053348 crossref_primary_10_1016_S0006_2952_00_00315_4 crossref_primary_10_1074_jbc_M113_533265 crossref_primary_10_1046_j_1471_4159_2001_00476_x crossref_primary_10_1074_jbc_M506799200 crossref_primary_10_1002_biot_200600092 crossref_primary_10_1083_jcb_200404142 crossref_primary_10_1016_j_yexcr_2007_09_010 crossref_primary_10_1078_0171_9335_00132 crossref_primary_10_1002_mc_20393 crossref_primary_10_1002_mrd_1080330105 crossref_primary_10_1042_BJ20040806 crossref_primary_10_1091_mbc_e07_05_0487 crossref_primary_10_1095_biolreprod64_4_1106 crossref_primary_10_1016_j_carpath_2007_01_004 crossref_primary_10_1006_meth_1999_0931 crossref_primary_10_1007_BF00370421 crossref_primary_10_1016_S0735_1097_01_01443_7 crossref_primary_10_1091_mbc_10_6_2033 crossref_primary_10_1016_0896_6273_93_90068_3 crossref_primary_10_1002__SICI_1098_1136_199809_24_1_97__AID_GLIA10_3_0_CO_2_T crossref_primary_10_1093_carcin_bgg066 crossref_primary_10_1242_jcs_113_12_2207 crossref_primary_10_1111_j_1432_1033_1997_00089_x crossref_primary_10_1006_meth_1999_0935 crossref_primary_10_1152_ajplung_00078_2002 crossref_primary_10_1242_jcs_185017 crossref_primary_10_1002_jimd_12264 crossref_primary_10_1002_bies_950180906 crossref_primary_10_1002__SICI_1096_9896_199812_186_4_343__AID_PATH189_3_0_CO_2_X crossref_primary_10_1016_S0306_4522_00_00032_4 crossref_primary_10_1016_j_yjmcc_2018_11_008 crossref_primary_10_1042_BJ20070550 crossref_primary_10_3390_ijms17020178 crossref_primary_10_1007_s12522_012_0130_0 crossref_primary_10_1002__SICI_1098_2744_199601_15_1_18__AID_MC4_3_0_CO_2_O crossref_primary_10_1016_0955_0674_90_90086_T crossref_primary_10_1046_j_1432_1327_1999_00502_x crossref_primary_10_1038_s41598_017_13488_9 crossref_primary_10_1074_jbc_M412612200 crossref_primary_10_1111_j_1349_7006_2002_tb01302_x crossref_primary_10_1242_jcs_00440 crossref_primary_10_1073_pnas_1803907115 crossref_primary_10_1152_ajpheart_01028_2001 crossref_primary_10_1186_1471_2474_2_10 crossref_primary_10_1002_jemt_1070270206 crossref_primary_10_1038_nrc2841 crossref_primary_10_1007_s00232_012_9444_4 crossref_primary_10_1242_dev_113_1_165 crossref_primary_10_1016_j_placenta_2003_08_013 crossref_primary_10_1080_15419060701402320 crossref_primary_10_1007_s00232_007_9035_y crossref_primary_10_1111_j_1432_1033_1991_tb21075_x crossref_primary_10_1152_ajpcell_00052_2002 crossref_primary_10_1095_biolreprod64_4_1255 crossref_primary_10_1007_s00210_011_0603_4 crossref_primary_10_1089_hum_1998_9_15_2253 crossref_primary_10_1074_jbc_M504401200 crossref_primary_10_1080_cac_10_4_6_215_220 crossref_primary_10_1046_j_1460_9568_1998_00253_x crossref_primary_10_1210_endo_142_1_7893 crossref_primary_10_1074_jbc_M201256200 crossref_primary_10_3390_biology11020283 crossref_primary_10_1002_jcp_20971 crossref_primary_10_1080_15419060802198736 crossref_primary_10_1002_hep_510300304 crossref_primary_10_1002__SICI_1097_4652_199801_174_1_66__AID_JCP8_3_0_CO_2_E crossref_primary_10_1074_jbc_M410548200 crossref_primary_10_1126_stke_2000_54_pe1 crossref_primary_10_1128_mcb_11_10_5364_5371_1991 crossref_primary_10_1016_j_cellsig_2017_01_008 crossref_primary_10_1002_glia_20435 crossref_primary_10_1016_j_tice_2023_102155 crossref_primary_10_1091_mbc_e04_01_0056 crossref_primary_10_1091_mbc_e10_05_0403 crossref_primary_10_1530_rep_1_00277 crossref_primary_10_1002__SICI_1520_6408_1999_24_1_2_91__AID_DVG10_3_0_CO_2 crossref_primary_10_1006_jsre_2001_6346 crossref_primary_10_1074_jbc_270_16_9281 crossref_primary_10_1089_hyb_2008_0082 crossref_primary_10_1146_annurev_cellbio_19_111301_144309 crossref_primary_10_1016_S0006_8993_00_01987_9 crossref_primary_10_1016_S0002_9378_94_70284_5 crossref_primary_10_1016_S0079_6107_05_80003_3 crossref_primary_10_1177_026119299502300410 crossref_primary_10_1083_jcb_144_5_1033 crossref_primary_10_1016_j_yexcr_2008_07_030 crossref_primary_10_1016_S0021_9258_18_54068_0 crossref_primary_10_1007_s00398_016_0106_1 crossref_primary_10_1111_j_1540_8167_1999_tb00645_x crossref_primary_10_1002_glia_440100404 crossref_primary_10_1096_fj_15_276527 crossref_primary_10_1152_ajpcell_2000_279_1_C147 crossref_primary_10_1359_jbmr_1999_14_10_1751 crossref_primary_10_1006_mcbr_2000_0200 crossref_primary_10_1078_0171_9335_00316 crossref_primary_10_1016_S0736_5748_99_00024_6 crossref_primary_10_1016_0898_6568_94_00090_X crossref_primary_10_1007_s00232_012_9453_3 crossref_primary_10_1074_jbc_M202652200 crossref_primary_10_1002__SICI_1098_2795_199707_47_3_231__AID_MRD1_3_0_CO_2_M crossref_primary_10_1016_S0014_5793_02_03441_5 crossref_primary_10_1091_mbc_e12_07_0537 crossref_primary_10_1007_BF01235758 crossref_primary_10_3109_10409238_2012_683482 crossref_primary_10_1152_jn_2002_88_4_1893 crossref_primary_10_1146_annurev_cellbio_13_1_119 crossref_primary_10_1242_jcs_113_17_3037 crossref_primary_10_1152_ajpcell_00413_2007 crossref_primary_10_1016_S0165_6147_98_01192_4 crossref_primary_10_1371_journal_pone_0109128 crossref_primary_10_1038_sj_emboj_7600605 crossref_primary_10_1093_cvr_cvy251 crossref_primary_10_1210_jcem_84_5_5644 crossref_primary_10_1242_jcs_103_1_81 crossref_primary_10_1007_s00441_010_0938_z crossref_primary_10_1078_S0171_9335_04_70030_8 crossref_primary_10_1016_S0002_9378_11_91453_7 crossref_primary_10_1002_glia_440050102 crossref_primary_10_1083_jcb_200105047 crossref_primary_10_1111_j_0953_816X_2004_03197_x crossref_primary_10_1016_S0092_8674_00_81282_9 crossref_primary_10_1016_0012_1606_92_90227_8 crossref_primary_10_1161_01_RES_85_11_1046 crossref_primary_10_1016_0092_8674_93_90728_9 crossref_primary_10_1002__SICI_1097_4644_19990901_74_3_349__AID_JCB4_3_0_CO_2_5 crossref_primary_10_1016_j_neuroscience_2009_01_080 crossref_primary_10_1074_jbc_M402006200 crossref_primary_10_1002_mc_2940100407 crossref_primary_10_1038_nsb0197_39 crossref_primary_10_1002__SICI_1097_0045_19990101_38_1_55__AID_PROS7_3_0_CO_2_4 crossref_primary_10_1002__SICI_1097_4652_199808_176_2_332__AID_JCP11_3_0_CO_2_5 crossref_primary_10_1074_jbc_270_44_26399 crossref_primary_10_1074_jbc_M113_454389 crossref_primary_10_1002_aja_1002040403 crossref_primary_10_1161_01_RES_0000091259_84556_D5 crossref_primary_10_1253_circj_71_973 crossref_primary_10_1161_CIRCRESAHA_110_223412 crossref_primary_10_1016_S0002_9440_10_64133_8 crossref_primary_10_1074_jbc_M003917200 crossref_primary_10_1111_j_1460_9568_2000_01331_x crossref_primary_10_1002_cne_21920 crossref_primary_10_1016_j_abb_2004_11_002 crossref_primary_10_1002__SICI_1520_6408_1999_24_1_2_111__AID_DVG11_3_0_CO_2_P crossref_primary_10_1593_neo_05847 crossref_primary_10_1007_BF00756491 crossref_primary_10_1242_jcs_00951 crossref_primary_10_1053_j_gastro_2007_03_029 crossref_primary_10_1161_01_ATV_19_7_1615 |
ContentType | Journal Article |
Copyright | Copyright 1990 The Rockefeller University Press 1991 INIST-CNRS |
Copyright_xml | – notice: Copyright 1990 The Rockefeller University Press – notice: 1991 INIST-CNRS |
DBID | IQODW CGR CUY CVF ECM EIF NPM AAYXX CITATION 7X8 5PM |
DOI | 10.1083/jcb.111.5.2077 |
DatabaseName | Pascal-Francis Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef MEDLINE - Academic PubMed Central (Full Participant titles) |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef MEDLINE - Academic |
DatabaseTitleList | MEDLINE CrossRef MEDLINE - Academic |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Biology |
EISSN | 1540-8140 |
EndPage | 2088 |
ExternalDocumentID | 10_1083_jcb_111_5_2077 2172261 19421380 1614268 |
Genre | Research Support, U.S. Gov't, P.H.S Journal Article |
GrantInformation_xml | – fundername: NICHD NIH HHS grantid: HD09635 – fundername: NIGMS NIH HHS grantid: GM-18974 – fundername: NEI NIH HHS grantid: EY-02430 |
GroupedDBID | --- -DZ -~X .55 .GJ 0VX 123 18M 1VV 29K 2WC 36B 3O- 4.4 53G 85S 9QQ AAUTI ABDNZ ABOCM ABPIV ABPPZ ABRJW ABZEH ACGFO ACGOD ACIWK ACKIV ACKOT ACNCT ACNKL ACPRK ACPVT ADBBV AENEX AEUPB AFFDN AFMIJ AFNDN AFOSN AFRAH AGHSJ AHJTV AI. ALMA_UNASSIGNED_HOLDINGS AOIJS B-7 BAWUL BTFSW C1A C45 CS3 D-I D0L DIK DU5 E3Z EBS EJD F20 F5P F9R FRP GX1 HF~ HGD HYE IH2 J5H JENOY JST JZ9 KQ8 MVM N9A O5R O5S OK1 P2P PQQKQ R.V RHF RHI RNS RPM RXW SJN TN5 TR2 TRP TWZ UHB UKR UPT VH1 VQA W8F WH7 WOQ X7L X7M XOL YNH YOC YQT YYP YZZ ZA5 ZCA ZGI ~KM 08R 34G 39C AAUGY ABPTK ABTAH ACYGS AGCDD AGJRR BKOMP EMB EMOBN H13 IQODW NHB OVD SV3 TAE TAF UBX YKV YSK YWH ZY4 AEILP CGR CUY CVF ECM EIF NPM AAYXX CITATION 7X8 5PM |
ID | FETCH-LOGICAL-c4177-69aa36df583bcc088e782768059d20177ca1f68cb1ef1784cc97f78583e7686c3 |
IEDL.DBID | RPM |
ISSN | 0021-9525 |
IngestDate | Tue Sep 17 21:03:21 EDT 2024 Fri Oct 25 22:58:55 EDT 2024 Thu Sep 26 16:00:14 EDT 2024 Sat Sep 28 08:36:39 EDT 2024 Sun Oct 29 17:07:16 EDT 2023 Fri Feb 02 07:01:37 EST 2024 |
IsDoiOpenAccess | true |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 5 |
Keywords | Immunohistochemistry Phosphorylation Gel electrophoresis Rodentia Biosynthesis Gap junction Posttranslational modification Proteins Vertebrata Immunoprecipitation reaction Mammalia Cell line Localization Pulse labelling |
Language | English |
License | CC BY 4.0 This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-c4177-69aa36df583bcc088e782768059d20177ca1f68cb1ef1784cc97f78583e7686c3 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
OpenAccessLink | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2116332/ |
PMID | 2172261 |
PQID | 80087574 |
PQPubID | 23479 |
PageCount | 12 |
ParticipantIDs | pubmedcentral_primary_oai_pubmedcentral_nih_gov_2116332 proquest_miscellaneous_80087574 crossref_primary_10_1083_jcb_111_5_2077 pubmed_primary_2172261 pascalfrancis_primary_19421380 jstor_primary_1614268 |
PublicationCentury | 1900 |
PublicationDate | 19901101 |
PublicationDateYYYYMMDD | 1990-11-01 |
PublicationDate_xml | – month: 11 year: 1990 text: 19901101 day: 1 |
PublicationDecade | 1990 |
PublicationPlace | New York, NY |
PublicationPlace_xml | – name: New York, NY – name: United States |
PublicationTitle | The Journal of cell biology |
PublicationTitleAlternate | J Cell Biol |
PublicationYear | 1990 |
Publisher | Rockefeller University Press The Rockefeller University Press |
Publisher_xml | – name: Rockefeller University Press – name: The Rockefeller University Press |
SSID | ssj0004743 |
Score | 1.9590663 |
Snippet | Connexin43 is a member of the highly homologous connexin family of gap junction proteins. We have studied how connexin monomers are assembled into functional... |
SourceID | pubmedcentral proquest crossref pubmed pascalfrancis jstor |
SourceType | Open Access Repository Aggregation Database Index Database Publisher |
StartPage | 2077 |
SubjectTerms | Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Cell Adhesion Molecules - physiology Cell communication Cell Communication - physiology Cell culture techniques Cell Line Cell lines Cells Cellular immunity Connexins Epithelial cells Fundamental and applied biological sciences. Psychology Gap junctions Hepatocytes Intercellular Junctions - chemistry Intercellular Junctions - physiology Membrane Proteins - biosynthesis Membrane Proteins - metabolism Miscellaneous Phosphorylation Protein Processing, Post-Translational Proteins Transfection |
Title | Differential Phosphorylation of the Gap Junction Protein Connexin43 in Junctional Communication-Competent and -Deficient Cell Lines |
URI | https://www.jstor.org/stable/1614268 https://www.ncbi.nlm.nih.gov/pubmed/2172261 https://search.proquest.com/docview/80087574 https://pubmed.ncbi.nlm.nih.gov/PMC2116332 |
Volume | 111 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1LT9wwEB6xSEhcKtqCmpZufajUU3bj2LGdI6KlqBJVD0XaW-RnWQTeFbtIcOaPM84D2KqnHnJwxo4Szzj-Rv5mBuAzIgjnjC9yl8hh3BiTm6LyuaFO17oOhfDpRPfspzg95z9m1WwLqiEWpiXtWzOfxKvrSZxftNzK5bWdDjyx6a-zY3RaBGPldAQjNNDBRR-CIWVPlUvcg6qshkyNik0vrUm_iEmFfqGUu7CTqjOVgm7sSR0tMXEk9QqnKXT1Lf4FQP_mUb7YmE724FWPKMlR9-avYcvHN7DT1Zi8fwsPX_sSKLiUr8jyYrHC6-a-o8CRRSCIAMkfvSSXuMO199rUDfNIbOLA3M0jZwRbgxgfYl8GleS2R95roqMjufMpJ0VqpjMBklDsah_OT779Pj7N-8oLueVUylzUWjPhQqWYsRZ_RB6BBDomiMUcIgYpraZBKGuoD1Qqbm0tg1TY3WMvYdkBbMdF9O-AoD_FTOkqp5zhhTG1CUajSShXcGODzeDLMPfNskuw0bQH44o1qK_koTRVk_SVwX6rmuduCC1KoTIYb6jqWV7zkjJVZPBp0F2Diyd9vY5-cbtqVJfQn2dw0GnyaWxvGRnIDRU_yVNW7k0JGmubnbs3zvf_PfID7FLECF3A4yFsr29u_UdEPmszhtH3GR239v4IUwcIIw |
link.rule.ids | 230,315,730,783,787,888,27936,27937,53804,53806 |
linkProvider | National Library of Medicine |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1LT9wwEB5RKlQuVR8g0gf4UKmn7CaxYzvHihZtWxb1ABK3yM-yCLwrdpHKuX-84zgBtuqphxycsaPEM46_kb-ZAfiACMJa7YrcRnIY01rnuqhdrkurGtX4grt4ojs94ZMz9u28Pt-AeoiF6Uj7Rs9G4ep6FGYXHbdycW3GA09s_GN6iE4Lp7QaP4GnuF4LNjjpQzik6MlykX1QV_WQq1HS8aXR8ScxqtEzFGIbtmJ9poqXa7tSIiZGlqRa4kT5VOHiXxD0byblo63p6AU87zEl-ZTe_SVsuPAKtlKVybvX8PtzXwQFF_MVWVzMl3jd3CUSHJl7ghiQ_FQLcol7XHevS94wC8REFsyvWWCUYGsQ40PM47CS3PTYe0VUsCS3LmaliM14KkAijl3uwNnRl9PDSd7XXsgNK4XIeaMU5dbXkmpj8FfkEEqga4JozCJmEMKo0nNpdOl8KSQzphFeSOzusBc3dBc2wzy4PSDoUVFd2dpKq1mhdaO9VmgU0hZMG28y-DjMfbtIKTba7mhc0hb1FX2Utm6jvjLY6VTz0A3BRcVlBvtrqnqQN6wq0UIyOBh01-LyiV-vgpvfLluZUvqzDHaTJu_H9paRgVhT8b085uVel6C5dvm5e_N8898jD-DZ5HR63B5_Pfn-FrZLRAwp_PEdbK5ubt17xEErvd9Z_R8fIQqI |
linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1LT9wwEB61VFRcqj5ATWnBh0o9ZfNwYjvHCrqiDxCHInGL_CyLwBuxi1TO_eOM4wR2q556yMEZO3I8Y_sb-fMMwEdEEMYom6cmkMMqpVSq8tqmqjCykY3LmQ0nuscn7Ois-nZen6-k-upJ-1rNJv7qeuJnFz23srvW2cgTy06PD9BpYZSWWWdc9hSe4ZzN2eioj1ci-UCYCwyEuqzHeI2CZpdahYViUqN3yPkWbIYcTSUr1namSE4MTEm5wMFyMcvFv2Do32zKle1p-hJeDLiSfI79fwVPrH8NmzHT5N0b-HM4JELBCX1Fuov5Ap-bu0iEI3NHEAeSX7Ijl7jP9e_6AA4zT3Rgwvye-YoSLI1i_IhevVqS6gF_L4n0hqTGhsgUoRhOBkjAsottOJt--XlwlA75F1JdFZynrJGSMuNqQZXWuBxZhBPoniAiM4gbONeycExoVVhXcFFp3XDHBVa3WItpugMbfu7tWyDoVVFVmtoIo6pcqUY5JdEwhMkrpZ1O4NM49m0Xw2y0_fG4oC3qK_gpbd0GfSWw3avmsRoCjJKJBPbWVPUob6qyQAtJYH_UXYtTKPy99HZ-u2hFDOtfJbATNfnQdrCMBPiaih_kITb3ugRNto_RPZjou_9uuQ_PTw-n7Y-vJ993YatA0BBvQL6HjeXNrf2AUGip9nqjvwfi0Qub |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Differential+Phosphorylation+of+the+Gap+Junction+Protein+Connexin43+in+Junctional+Communication-Competent+and+-Deficient+Cell+Lines&rft.jtitle=The+Journal+of+cell+biology&rft.au=Musil%2C+Linda+S.&rft.au=Cunningham%2C+Bruce+A.&rft.au=Edelman%2C+Gerald+M.&rft.au=Goodenough%2C+Daniel+A.&rft.date=1990-11-01&rft.pub=Rockefeller+University+Press&rft.issn=0021-9525&rft.eissn=1540-8140&rft.volume=111&rft.issue=5&rft.spage=2077&rft.epage=2088&rft_id=info:doi/10.1083%2Fjcb.111.5.2077&rft.externalDocID=1614268 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0021-9525&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0021-9525&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0021-9525&client=summon |