Detection of Strawberry crinkle virus in plants and aphids by RT‐PCR using conserved L gene sequences

• Published in: Plant pathology Vol. 51; no. 3; pp. 266 - 274
• Main Authors: Posthuma, K. I., Adams, A. N., Hong, Y., Kirby, M. J.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01.06.2002; Blackwell; Wiley Subscription Services, Inc
• Subjects: Biological and medical sciences; Fragaria; Fundamental and applied biological sciences. Psychology; Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control; Microbiology; Mononegavirales; Phytopathology. Animal pests. Plant and forest protection; Plant viruses and viroids; Rhabdoviridae; RNA‐dependent RNA polymerase; strawberry; Strawberry crinkle virus; Techniques used in virology; Virology; Plant pathology; Horticulture; Plant pathogen; RNA; Entomology; Small fruits; Insecta; Rosaceae; Transmission; Conserved sequence; Pest; Cytorhabdovirus; Homoptera; Gene; Rhabdoviridae; Dicotyledones; Host plant; Angiospermae; Aphididae; Strawberry crinkle virus; Solanaceae; Reverse transcription polymerase chain reaction; Sequencing; Vector; Nucleic acid; Method study; Chaetosiphon fragaefolii; Aphidoidea; Fragaria; Enzyme; Physalis; Transferases; Experimental study; RNA-directed RNA polymerase; Virology; Infection; Virus; Mononegavirales; Nucleotidyltransferases; Analysis method; Complementary DNA; Fruit crop; Arthropoda; Viral disease; Spermatophyta; Molecular biology; Invertebrata; Detection

About 10% of the large (L) protein gene of Strawberry crinkle virus (SCV) was sequenced after amplification with degenerate primers designed to conserved regions of the rhabdovirus L protein. The virus sequence was extended to 1362 nucleotides through rapid amplification of cDNA ends. One pair of de...