Segmenting Microscopy Images of Multi-Well Plates Based on Image Contrast

• Published in: Microscopy and microanalysis Vol. 23; no. 5; pp. 932 - 937
• Main Authors: Chen, Weiyang, Liao, Bo, Li, Weiwei, Dong, Xiangjun, Flavel, Matthew, Jois, Markandeya, Li, Guojun, Xian, Bo
• Format: Journal Article
• Language: English
• Published: New York, USA Cambridge University Press 01.10.2017; Oxford University Press
• Subjects: Aging; Bioinformatics; Biological activity; Biology; Bright plating; Caenorhabditis elegans; Experiments; Health care; Illumination; Image contrast; Image detection; Image processing; Image segmentation; Informatics; Instrumentation and Software; Methods; Microscopy; Nematodes; Software; bright field; uneven illumination; image segmentation; microscopy image; multi-well plate

Image segmentation is a key process in analyzing biological images. However, it is difficult to detect the differences between foreground and background when the image is unevenly illuminated. The unambiguous segmenting of multi-well plate microscopy images with various uneven illuminations is a cha...