Lectin from Dioclea violacea induces autophagy in U87 glioma cells

• Published in: International journal of biological macromolecules Vol. 134; pp. 660 - 672
• Main Authors: Nascimento, Ana Paula M., Wolin, Ingrid A.V., Welter, Priscilla G., Heinrich, Isabella A., Zanotto-Filho, Alfeu, Osterne, Vinicius J.S., Lossio, Claudia F., Silva, Mayara T.L., Nascimento, Kyria S., Cavada, Benildo S., Leal, Rodrigo B.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.08.2019
• Subjects: Animals; Astrocytes - drug effects; Astrocytes - metabolism; Autophagy; Autophagy - drug effects; Caspase 3 - metabolism; Cell Line, Tumor; Cell Movement - drug effects; Cell Survival - drug effects; Dioclea - chemistry; Dioclea violacea; Glioma - genetics; Glioma - metabolism; Humans; Membrane Potential, Mitochondrial - drug effects; Mitochondria - metabolism; mTOR; Plant Lectins - chemistry; Plant Lectins - isolation & purification; Plant Lectins - pharmacology; Rats; Reactive Oxygen Species - metabolism; Signal Transduction - drug effects; mTOR; Dioclea violacea; Autophagy

The antitumor activity of DVL, a lectin purified from Dioclea violacea seeds, on the U87 human glioma cell line was evaluated and compared with Canavalia ensiformis lectin (ConA). Treatment with DVL (10–100 μg/mL; 24–96 h) induced alterations in cell morphology, decreased cell numbers and clonogenic...