Cingulin and paracingulin tether myosins-2 to junctions to mechanoregulate the plasma membrane

• Published in: The Journal of cell biology Vol. 222; no. 7; p. 1
• Main Authors: Rouaud, Florian, Huang, Wenmao, Flinois, Arielle, Jain, Kunalika, Vasileva, Ekaterina, Di Mattia, Thomas, Mauperin, Marine, Parry, David A D, Dugina, Vera, Chaponnier, Christine, Méan, Isabelle, Montessuit, Sylvie, Mutero-Maeda, Annick, Yan, Jie, Citi, Sandra
• Format: Journal Article
• Language: English
• Published: United States Rockefeller University Press 03.07.2023
• Subjects: Accumulation; Actin; Actomyosin; Adherens Junctions - metabolism; Binding; Biophysics; Cell Membrane - metabolism; Cytoskeletal Proteins - metabolism; Cytoskeleton; Cytoskeleton - metabolism; Filaments; Isoforms; Localization; Membranes; Myosin; Myosins - metabolism; Phalloidin; Proteins; Stiffness; Tight Junctions - metabolism; Tortuosity; Zonula occludens-1 protein

The mechanisms that regulate the spatial sorting of nonmuscle myosins-2 (NM2) isoforms and couple them mechanically to the plasma membrane are unclear. Here we show that the cytoplasmic junctional proteins cingulin (CGN) and paracingulin (CGNL1) interact directly with NM2s through their C-terminal c...