Carbon and pH Modulate the Expression of the Fungal Glucose Repressor Encoding Genes

• Published in: Current microbiology Vol. 46; no. 2; pp. 146 - 150
• Main Authors: Vautard-Mey, Géraldine, Fèvre, Michel
• Format: Journal Article
• Language: English
• Published: New York, NY Springer 01.02.2003; Springer Nature B.V
• Subjects: Ascomycota; Ascomycota - genetics; Ascomycota - growth & development; Ascomycota - metabolism; Aspergillus nidulans; Aspergillus nidulans - genetics; Aspergillus nidulans - growth & development; Aspergillus nidulans - metabolism; Biological and medical sciences; carbon; Carbon - metabolism; Carbon sources; Cell Wall; Cell Wall - metabolism; chemistry; Culture Media; DNA-Binding Proteins; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; environmental factors; Enzymes; Fundamental and applied biological sciences. Psychology; Fungal Proteins; Fungal Proteins - chemistry; Fungal Proteins - genetics; Fungal Proteins - metabolism; Fungi; Gene expression; Gene Expression Regulation, Fungal; genetics; Glucose; growth & development; Growth, nutrition, metabolism, transports, enzymes. Molecular biology; Hydrogen-Ion Concentration; metabolism; Microbiology; Mycology; Oxalic acid; plant pathogenic fungi; Plant pathology; promoter regions; Promoter Regions, Genetic; Repressor Proteins; Repressor Proteins - chemistry; Repressor Proteins - genetics; Repressor Proteins - metabolism; Sclerotinia sclerotiorum; transcription (genetics); Transcription, Genetic; Fungi; Regulation(control); Plant pathogen; Gene; Sclerotinia sclerotiorum; pH; Ascomycetes; Glucose; Thallophyta; Catabolic repression
• DOI: 10.1007/s00284-002-3838-y
• ISSN: 0343-8651

The phytopathogenic necrotrophic fungus Sclerotinia sclerotiorum secretes large amounts of oxalic acid and cell wall-degrading enzymes (CWDE). Enzyme synthesis is pH dependent and subject to carbon catabolite repression. Because of the possible involvement of the glucose repressor CRE1 in the transcriptional control of the CWDE encoding genes, we studied crel expression with respect to the nature of the extracellular carbon source and the value of the external pH. Comparative analysis of the cre1 homologous gene creA from Aspergillus nidulans was performed with pH-deregulated mutants. The data obtained showed that ambient pH controls the expression level of the carbon repressor genes, and this control does not depend on the pH transcription factor PacC.