Histones participate in base excision repair of 8-oxodGuo by transiently cross-linking with active repair intermediates in nucleosome core particles

• Published in: Nucleic acids research Vol. 49; no. 1; pp. 257 - 268
• Main Authors: Ren, Mengtian, Shang, Mengdi, Wang, Huawei, Xi, Zhen, Zhou, Chuanzheng
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 11.01.2021
• Subjects: 8-Hydroxy-2'-Deoxyguanosine - metabolism; DNA Glycosylases - metabolism; DNA Repair - physiology; DNA-(Apurinic or Apyrimidinic Site) Lyase - metabolism; Genome Integrity, Repair and; Histones - physiology; Humans; Models, Molecular; Nucleic Acid Conformation; Nucleosomes - metabolism; Nucleosomes - ultrastructure; Phosphorus-Oxygen Lyases - metabolism; Protein Conformation; Ribosemonophosphates - metabolism

Abstract 8-Oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodGuo) is a biomarker of oxidative DNA damage and can be repaired by hOGG1 and APE1 via the base excision repair (BER) pathway. In this work, we studied coordinated BER of 8-oxodGuo by hOGG1 and APE1 in nucleosome core particles and found that histon...