Workflow for a Functional Assay of Candidate Effectors From Phytopathogens Using a TMV-GFP-based System

• Published in: Bio-protocol Vol. 15; no. 1369; p. e5287
• Main Authors: Cao, Peng, Shi, Haotian, Chen, Jialan, Cui, Langjun, Zhang, Meixiang, An, Yuyan
• Format: Journal Article
• Language: English
• Published: United States Bio-Protocol 20.04.2025; Bio-protocol LLC
• Subjects: Biology; Methods; Functional screening; Avirulence; TMV-GFP vector; Virulence; Effectors

The ability to efficiently screen plant pathogen effectors is crucial for understanding plant-pathogen interactions and developing disease-resistant crops. Traditional methods are often labor-intensive and time-consuming. Here, we present a robust, high-throughput screening assay using the tobacco m...