The Developmentally Regulated Avian Ch21 Lipocalin Is an Extracellular Fatty Acid-binding Protein

• Published in: The Journal of biological chemistry Vol. 271; no. 33; pp. 20163 - 20169
• Main Authors: Cancedda, Fiorella Descalzi, Malpeli, Mara, Gentili, Chiara, Di Marzo, Vincenzo, Bet, Paola, Carlevaro, Mariella, Cermelli, Silvia, Cancedda, Ranieri
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 16.08.1996; American Society for Biochemistry and Molecular Biology
• Subjects: Animals; Avian Proteins; Carrier Proteins - immunology; Carrier Proteins - metabolism; Chickens; Extracellular Space - chemistry; Fatty Acid-Binding Proteins; Fatty Acids - metabolism; Kinetics; Lipocalins; Molecular Weight; Myelin P2 Protein - metabolism; Neoplasm Proteins; Nucleopolyhedroviruses; Recombinant Proteins; Spodoptera

Ch21, a developmentally regulated extracellular protein expressed in chick embryos and in cultured chondrocytes, was expressed in the baculovirus system, and the recombinant protein was purified to homogeneity by gel-filtration chromatography. Separation of two isoforms was achieved on an ion-exchan...