Impact of assay design on test performance: lessons learned from 25-hydroxyvitamin D

• Published in: Clinical chemistry and laboratory medicine Vol. 52; no. 11; pp. 1579 - 1587
• Main Authors: Farrell, Christopher-John L., Soldo, Joshua, McWhinney, Brett, Bandodkar, Sushil, Herrmann, Markus
• Format: Journal Article
• Language: English
• Published: Germany De Gruyter 01.11.2014
• Subjects: 25-hydroxyvitamin D; 25-Hydroxyvitamin D 2 - blood; 25-Hydroxyvitamin D 2 - immunology; 25-Hydroxyvitamin D 2 - isolation & purification; Antibodies - immunology; Automation; Chromatography, High Pressure Liquid; Cross Reactions; Humans; immunoassay; Immunoassay - instrumentation; Immunoassay - methods; liquid chromatography-tandem mass spectrometry; Protein Binding; Tandem Mass Spectrometry; Vitamin D - analogs & derivatives; Vitamin D - blood; Vitamin D - immunology; Vitamin D - isolation & purification; Vitamin D-Binding Protein - chemistry; Vitamin D-Binding Protein - metabolism

Current automated immunoassays vary significantly in many aspects of their design. This study sought to establish if the theoretical advantages and disadvantages associated with different design formats of automated 25-hydroxyvitamin D (25-OHD) assays are translated into variations in assay performa...