Sortase A Fusion Expression and mIFc2 Co-Expression of Bovine Lactoferricin and Analysis of Its Antibacterial Activity

• Published in: Processes Vol. 10; no. 12; p. 2470
• Main Authors: Hsu, Chao-Yu, Hsieh, Chung-Yiu, Yang, Cheng-Yao, Chang, Yu-Kang, Shih, Wen-Ling, Yeh, Chuan-Ming, Hu, Nien-Jen, Chen, Ming-Shan, Nielsen, Brent L., Liu, Hung-Jen
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 22.11.2022
• Subjects: Amino acids; Analysis; Animals; Antibacterial activity; Antibacterial agents; Antibiotics; Antimicrobial peptides; antimicrobial peptides; lactoferricin B; staphylococcus aureus transferase SrtA fusion expression; mIFc2 co-expression; Bacteria; Bacterial infections; Biological response modifiers; Calcium ions; Cleavage; Cloning; E coli; Enzymes; Escherichia coli; Fusion protein; Gene order; Genetic engineering; Gram-negative bacteria; Gram-positive bacteria; Heat treatment; Inclusion bodies; Industrial research; N-Terminus; Peptides; pH effects; Plasmids; Proteins; R&D; Research & development; Sortase; Staphylococcus aureus; Toxicity; Vectors (Biology); γ-Interferon; New York; United States; Taiwan; United States > US

The coding region for the sortase A (SrtA) of Staphylococcus aureus was fused at the N-terminus of LfcinB. The SrtA-LfcinB fusion protein in E. coli C43(DE3) was expressed with the expected sizes of 21 kDa and 38 kDa by pET21b-SrtA-LfcinB and pET32-1SrtA-LfcinB constructs, respectively. Increased le...