Chemical Biology Approaches to Genome Editing: Understanding, Controlling, and Delivering Programmable Nucleases

• Published in: Cell chemical biology Vol. 23; no. 1; pp. 57 - 73
• Main Authors: Hu, Johnny H., Davis, Kevin M., Liu, David R.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Ltd 21.01.2016
• Subjects: Animals; Base Sequence; Clustered Regularly Interspaced Short Palindromic Repeats; CRISPR-Cas Systems; Deoxyribonucleases - genetics; Deoxyribonucleases - metabolism; DNA - genetics; DNA - metabolism; Genetic Engineering - methods; Genome; Humans

Programmable DNA nucleases have provided scientists with the unprecedented ability to probe, regulate, and manipulate the human genome. Zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and the clustered regularly interspaced short palindromic repeat-Cas9 system...