Combination of baculovirus-mediated gene delivery and packed-bed reactor for scalable production of adeno-associated virus

• Published in: Human gene therapy Vol. 18; no. 11; p. 1161
• Main Authors: Huang, Kuo-Shiang, Lo, Wen-Hsin, Chung, Yao-Chi, Lai, Yiu-Kay, Chen, Chi-Yuan, Chou, Szu-Ting, Hu, Yu-Chen
• Format: Journal Article
• Language: English
• Published: United States 01.11.2007
• Subjects: Animals; Baculoviridae - genetics; Bioreactors - economics; Cell Line; Dependovirus - genetics; Dependovirus - growth & development; Gene Transfer Techniques; Humans; Insecta - cytology; Recombinant Proteins - genetics; Virus Cultivation - methods; Virus Replication

The production of recombinant adeno-associated virus (rAAV) commonly requires plasmid cotransfection, which hinders its mass production. Herein we describe the development of a novel process for rAAV production by combining the advantages of baculovirus-mediated gene delivery and BelloCell bioreacto...