Delineation of Transmembrane Domains of the Na+/H+ Exchanger That Confer Sensitivity to Pharmacological Antagonists

• Published in: The Journal of biological chemistry Vol. 271; no. 33; pp. 19922 - 19927
• Main Authors: Orlowski, John, Kandasamy, Ramani A.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 16.08.1996; American Society for Biochemistry and Molecular Biology
• Subjects: Amiloride - analogs & derivatives; Amiloride - pharmacology; Animals; Biological Transport; Cimetidine - pharmacology; DNA, Complementary - genetics; Enzyme Inhibitors - pharmacology; Guanidines - pharmacology; Kinetics; Ligands; Membrane Glycoproteins - chemistry; Rats; Recombinant Fusion Proteins; Sodium-Hydrogen Exchanger 3; Sodium-Hydrogen Exchangers - antagonists & inhibitors; Structure-Activity Relationship; Sulfones - pharmacology

Plasma membrane Na+/H+ exchanger (NHE) isoforms NHE1 and NHE3 exhibit very different sensitivities to amiloride and its 5-amino-substituted analogues, benzoyl guanidinium derivatives (e.g. (3-methylsulfonyl-4-piperidinobenzoyl)guanidine methanesulfonate (HOE694)), and cimetidine. To define structura...