Multiplexed quantification of 63 proteins in human urine by multiple reaction monitoring-based mass spectrometry for discovery of potential bladder cancer biomarkers

• Published in: Journal of proteomics Vol. 75; no. 12; pp. 3529 - 3545
• Main Authors: Chen, Yi-Ting, Chen, Hsiao-Wei, Domanski, Dominik, Smith, Derek S., Liang, Kung-Hao, Wu, Chih-Ching, Chen, Chien-Lun, Chung, Ting, Chen, Min-Chi, Chang, Yu-Sun, Parker, Carol E., Borchers, Christoph H., Yu, Jau-Song
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 27.06.2012
• Subjects: adiponectin; Amino Acid Sequence; apolipoprotein A-II; Biomarker verification; biomarkers; Biomarkers, Tumor; Biomarkers, Tumor - chemistry; Biomarkers, Tumor - urine; Bladder cancer; bladder diseases; chemistry; complement; diagnosis; Feasibility Studies; ferroxidase; genetic variation; genome; hematuria; hernia; Humans; mass spectrometry; Mass Spectrometry - methods; methods; Molecular Sequence Data; Multiple reaction monitoring; Multiplexed quantitation; Neoplasm Proteins; Neoplasm Proteins - chemistry; Neoplasm Proteins - urine; patients; Peptide Mapping; Peptide Mapping - methods; prothrombin; Reproducibility of Results; Sensitivity and Specificity; urinary bladder neoplasms; Urinary Bladder Neoplasms - diagnosis; Urinary Bladder Neoplasms - urine; Urinary proteome; Urinary tract infection/hematuria; urine; LOD; Urinary tract infection/hematuria; Multiple reaction monitoring; NT; APOA2; APOA1; MRM; UTI; HU; LC–MS/MS; LgEs; Bladder cancer; Multiplexed quantitation; HgEs; LOQ; CV; HgAs; XIC; Biomarker verification; Urinary proteome; SIS

Three common urological diseases are bladder cancer, urinary tract infection, and hematuria. Seventeen bladder cancer biomarkers were previously discovered using iTRAQ — these findings were verified by MRM-MS in this current study. Urine samples from 156 patients with hernia (n=57, control), bladder...