Functional analysis of NTF-1, a developmentally regulated Drosophila transcription factor that binds neuronal cis elements

• Published in: Genes & development Vol. 3; no. 11; pp. 1677 - 1688
• Main Authors: DYNLACHT, B. D, ATTARDI, L. D, ADMON, A, FREEMAN, M, TJIAN, R
• Format: Journal Article
• Language: English
• Published: Cold Spring Harbor, NY Cold Spring Harbor Laboratory 01.11.1989
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Biological and medical sciences; Blotting, Northern; Cloning, Molecular; Diptera; DNA - metabolism; DNA-Binding Proteins - genetics; DNA-Binding Proteins - isolation & purification; DNA-Binding Proteins - metabolism; Drosophila - genetics; Drosophila Proteins; Drosophilidae; Fundamental and applied biological sciences. Psychology; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Neurons - metabolism; Nucleic Acid Hybridization; Promoter Regions, Genetic; Regulatory Sequences, Nucleic Acid; Transcription Factors - genetics; Transcription Factors - isolation & purification; Transcription Factors - metabolism; Transcription, Genetic; Transcription. Transcription factor. Splicing. Rna processing; Vaccinia virus - genetics; In vitro transcription; Embryonic development; Molecular hybridization; Purification; Insecta; Drosophila; In situ; Footprinting technique; DNA binding protein; Tissue specificity; Gene expression; Drosophilidae; Northern blotting; Arthropoda; DNA; Regulatory sequence; Invertebrata; Molecular cloning; Diptera; Aminoacid sequence

In an effort to characterize sequence-specific transcription factors that regulate gene expression during Drosophila development, we identified and purified a novel DNA-binding activity (NTF-1). The purified protein consists of several polypeptides that bind selectively to a functionally important c...