Lipopolysaccharide patterns in SDS-PAGE of rhizobia that nodulate leguminous trees

• Published in: FEMS microbiology letters Vol. 107; no. 2-3; pp. 327 - 330
• Main Authors: Lindstroem, K. (Helsinki Univ. (Finland). Dept. of Applied Chemistry and Microbiology), Zahran, H.H
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.03.1993; Blackwell
• Subjects: Acacia; ACACIA SENEGAL; Bacteriology; Biological and medical sciences; FORMATION DE NODOSITES; Fundamental and applied biological sciences. Psychology; LIPOPOLISACARIDOS; LIPOPOLYSACCHARIDE; LIPOPOLYSACCHARIDES; Microbiology; Morphology, structure, chemical composition; NODULACION; Prosopin; PROSOPIS CHILENSIS; RHIZOBIUM; ROOT NODULATION; SDS‐PAGE; Prosopis chilensis; Gel electrophoresis; Rhizobium; Leguminosae; Dicotyledones; Acacia senegal; Angiospermae; Lipopolysaccharide; Bacteria; Isolate; Spermatophyta; Rhizobiaceae; Comparative study
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1111/j.1574-6968.1993.tb06052.x

Lipopolysaccharide (LPS) patterns were obtained from fast‐growing Rhizobium strains after silver staining of proteinase K treated cells lysates, run in SDS‐PAGE. The rhizobia came from root nodules of Acacia senegal and Prosopis chilensis, collected in differents part of the Sudan. The LPS profiles of all strains were typical of rhizobia. Two different LPS region with lower and higher electrophoretic mobility (region I and region II, respectively) coulld be dinguished in the gels, and based on the profiles the strains were divided into three groups. Strains isolated from A. senegal showed a wider range of different profiles than strains isolated from P. chilensis, even though the plants belong to the same cross‐infection group. Otherwise there was no clear correlation between the taxonomic relatedness or site of isolation of the strains and their LPS profiles.