The principal site of nonenzymatic glycosylation of human serum albumin in vivo

• Published in: The Journal of biological chemistry Vol. 258; no. 10; pp. 6142 - 6146
• Main Authors: Garlick, R L, Mazer, J S
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 25.05.1983; American Society for Biochemistry and Molecular Biology
• Subjects: Amino Acids - analysis; Borohydrides; Chromatography, Affinity; Chromatography, Gel; Glucose - metabolism; Humans; Peptide Fragments - isolation & purification; Serum Albumin - analysis; Serum Albumin - metabolism; Trypsin

We have determined the major site of nonenzymatic glycosylation of human serum albumin in vivo. This was accomplished by reacting freshly purified human serum albumin with sodium [3H]borohydride followed by aminoethylation and tryptic digestion. The tryptic peptides were separated into a soluble fra...