Identification of auxotrophic mutants of the yeast Kluyveromyces marxianus by non‐homologous end joining‐mediated integrative transformation with genes from Saccharomyces cerevisiae

• Published in: Yeast (Chichester, England) Vol. 30; no. 12; pp. 485 - 500
• Main Authors: Yarimizu, Tohru, Nonklang, Sanom, Nakamura, Junpei, Tokuda, Shuya, Nakagawa, Takaaki, Lorreungsil, Sasithorn, Sutthikhumpha, Surasit, Pukahuta, Charida, Kitagawa, Takao, Nakamura, Mikiko, Cha‐aim, Kamonchai, Limtong, Savitree, Hoshida, Hisashi, Akada, Rinji
• Format: Journal Article
• Language: English
• Published: England Wiley Subscription Services, Inc 01.12.2013
• Subjects: auxotrophic mutants; DNA, Fungal - genetics; Genetic Complementation Test; Kluyveromyces - cytology; Kluyveromyces - genetics; Kluyveromyces marxianus; mating; Mutation; non‐homologous end‐joining; Plasmids - genetics; Recombination, Genetic; Saccharomyces cerevisiae; Saccharomyces cerevisiae - cytology; Saccharomyces cerevisiae - genetics; tetrad; Transformation, Genetic; Transgenes; Kluyveromyces marxianus; non-homologous end-joining; auxotrophic mutants; tetrad; Saccharomyces cerevisiae; mating

The isolation and application of auxotrophic mutants for gene manipulations, such as genetic transformation, mating selection and tetrad analysis, form the basis of yeast genetics. For the development of these genetic methods in the thermotolerant fermentative yeast Kluyveromyces marxianus, we isola...