Isolation and characterization of bacteriocin‐producing bacteria from the intestinal microbiota of elderly Irish subjects

• Published in: Journal of applied microbiology Vol. 114; no. 3; pp. 886 - 898
• Main Authors: Lakshminarayanan, B., Guinane, C.M., O'Connor, P.M., Coakley, M., Hill, C., Stanton, C., O'Toole, P.W., Ross, R.P.
• Format: Journal Article
• Language: English
• Published: Oxford Blackwell 01.03.2013; Oxford University Press
• Subjects: 16S rDNA gene amplicon sequencing; Aged; Bacteria; Bacterial Proteins - isolation & purification; bacteriocins; Bacteriocins - biosynthesis; Bacteriocins - isolation & purification; Biological and medical sciences; DNA Fingerprinting; DNA, Bacterial - genetics; elderly; Enterococcus; Enterococcus - genetics; Enterococcus - isolation & purification; Enterococcus - metabolism; Enterococcus faecium; Feces; Feces - microbiology; Fundamental and applied biological sciences. Psychology; Humans; Indicator species; intestinal microbiota; Intestines - microbiology; Ireland; Lactobacillus - genetics; Lactobacillus - isolation & purification; Lactobacillus - metabolism; Lactobacillus bulgaricus; Lactobacillus spp; Lactococcus lactis; Listeria innocua; Microbiology; Microbiota; Microorganisms; Older people; Pathology; Probiotics; RNA, Ribosomal, 16S - genetics; Streptococcus mutans; Ireland; Human; Lactic acid bacteria; Applied microbiology; 16S rDNA gene amplicon sequencing; Bacteriocin; Ribosomal DNA; Gut; bacteriocins; Microflora; Characterization; intestinal microbiota; Gene; Lactobacillus spp; 16S-RNA; Bacteria; Lactobacillaceae; Isolation; Elderly; Sequencing; Lactobacillus

Aims To isolate and characterize bacteriocins produced by predominant species of lactic acid bacteria from faeces of elderly subjects. Methods and Results Screening over 70 000 colonies, from faecal samples collected from 266 subjects, using the indicator organisms Lactobacillus bulgaricus LMG 6901...