The diffusible factor synthase XanB2 is a bifunctional chorismatase that links the shikimate pathway to ubiquinone and xanthomonadins biosynthetic pathways

• Published in: Molecular microbiology Vol. 87; no. 1; pp. 80 - 93
• Main Authors: Zhou, Lian, Wang, Jia‐Yuan, Wang, Jianhe, Poplawsky, Alan, Lin, Shuangjun, Zhu, Bangshang, Chang, Changqing, Zhou, Tielin, Zhang, Lian‐Hui, He, Ya‐Wen
• Format: Journal Article
• Language: English
• Published: Oxford Blackwell 01.01.2013; Blackwell Publishing Ltd
• Subjects: Anisoles - metabolism; Bacteria; Biological and medical sciences; Biosynthesis; Biosynthetic Pathways - genetics; Carbon-Oxygen Lyases - metabolism; Chorismic Acid - metabolism; Enzyme kinetics; Frateuria; Fundamental and applied biological sciences. Psychology; Gene Deletion; Hydroxybenzoates - metabolism; Microbiology; Nonnative species; Shikimic Acid - metabolism; Ubiquinone - metabolism; Variovorax; Xanthomonas; Xanthomonas campestris; Xanthomonas campestris - enzymology; Xanthomonas campestris - genetics; Xanthomonas campestris - metabolism; Xylophilus; Ubiquinone; Synthase; Antioxidant; Enzyme

Summary The diffusible factor synthase XanB2, originally identified in Xanthomonas campestris pv. campestris (Xcc), is highly conserved across a wide range of bacterial species, but its substrate and catalytic mechanism have not yet been investigated. Here, we show that XanB2 is a unique bifunctiona...