High-throughput nucleoside phosphate monitoring in mammalian cell fed-batch cultivation using quantitative matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

• Published in: Biotechnology journal Vol. 10; no. 1; p. 190
• Main Authors: Steinhoff, Robert F, Ivarsson, Marija, Habicher, Tobias, Villiger, Thomas K, Boertz, Jens, Krismer, Jasmin, Fagerer, Stephan R, Soos, Miroslav, Morbidelli, Massimo, Pabst, Martin, Zenobi, Renato
• Format: Journal Article
• Language: English
• Published: Germany 01.01.2015
• Subjects: Adenosine Triphosphate - metabolism; Animals; Cell Culture Techniques - methods; Cell Line; Cell Survival; Intracellular Space - metabolism; Metabolomics - methods; Mice; Principal Component Analysis; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods; Microarrays for mass spectrometry (MAMS); Automated sample aliquoting; MALDI-MS; Monoclonal antibody production; ATP

Current methods for monitoring multiple intracellular metabolite levels in parallel are limited in sample throughput capabilities and analyte selectivity. This article presents a novel high-throughput method based on matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometr...