A new method for tracking poultry litter in the Potomac Basin headwaters of West Virginia
Aim To validate the distribution of a poultry litter‐specific marker gene in faecally contaminated environmental waters of an intensive poultry litter rearing region. Methods and Results A TaqMan®‐based qPCR assay for Brevibacterium sp. LA35 16S rRNA (LA35 gene), which was previously shown to be ass...
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Published in | Journal of applied microbiology Vol. 115; no. 2; pp. 445 - 454 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Oxford
Blackwell
01.08.2013
Oxford University Press |
Subjects | |
Online Access | Get full text |
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Abstract | Aim
To validate the distribution of a poultry litter‐specific marker gene in faecally contaminated environmental waters of an intensive poultry litter rearing region.
Methods and Results
A TaqMan®‐based qPCR assay for Brevibacterium sp. LA35 16S rRNA (LA35 gene), which was previously shown to be associated with poultry litter and faeces, was tested on 126 nontarget faecal samples and 28 poultry litter and faecal samples. The TaqMan assay was sensitive (76%) and specific (100%) to the LA35 gene and exhibited a detection limit for poultry litter in water samples that is sufficiently low (2·5 × 10−2 mg litter l−1) to be applicable for environmental monitoring. The LA35 gene was detected in 43% of water samples (n = 30) collected in an intensive poultry rearing region of West Virginia which drains to the Chesapeake Bay.
Conclusions
The poultry‐specific TaqMan qPCR method for the LA35 gene is more specific than previously published methods and can be used to identify regions impacted by poultry rearing activities.
Significance and Impact of the Study
The LA35 gene appears to have a broad geographical distribution as it has been found in poultry litter and faeces from Delaware and West Virginia, in this study and from Arkansas, Georgia, Florida, Minnesota, Oklahoma and Utah previously. |
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AbstractList | To validate the distribution of a poultry litter-specific marker gene in faecally contaminated environmental waters of an intensive poultry litter rearing region. A TaqMan registered -based qPCR assay for Brevibacterium sp. LA35 16S rRNA (LA35 gene), which was previously shown to be associated with poultry litter and faeces, was tested on 126 nontarget faecal samples and 28 poultry litter and faecal samples. The TaqMan assay was sensitive (76%) and specific (100%) to the LA35 gene and exhibited a detection limit for poultry litter in water samples that is sufficiently low (2.5 x 10 super(-2) mg litter l super(-1)) to be applicable for environmental monitoring. The LA35 gene was detected in 43% of water samples (n = 30) collected in an intensive poultry rearing region of West Virginia which drains to the Chesapeake Bay. The poultry-specific TaqMan qPCR method for the LA35 gene is more specific than previously published methods and can be used to identify regions impacted by poultry rearing activities. The LA35 gene appears to have a broad geographical distribution as it has been found in poultry litter and faeces from Delaware and West Virginia, in this study and from Arkansas, Georgia, Florida, Minnesota, Oklahoma and Utah previously. Aim To validate the distribution of a poultry litter-specific marker gene in faecally contaminated environmental waters of an intensive poultry litter rearing region. Methods and Results A TaqMan-based qPCR assay for Brevibacterium sp. LA35 16S rRNA (LA35 gene), which was previously shown to be associated with poultry litter and faeces, was tested on 126 nontarget faecal samples and 28 poultry litter and faecal samples. The TaqMan assay was sensitive (76%) and specific (100%) to the LA35 gene and exhibited a detection limit for poultry litter in water samples that is sufficiently low (2·5 × 10-2 mg litter l-1) to be applicable for environmental monitoring. The LA35 gene was detected in 43% of water samples (n = 30) collected in an intensive poultry rearing region of West Virginia which drains to the Chesapeake Bay. Conclusions The poultry-specific TaqMan qPCR method for the LA35 gene is more specific than previously published methods and can be used to identify regions impacted by poultry rearing activities. Significance and Impact of the Study The LA35 gene appears to have a broad geographical distribution as it has been found in poultry litter and faeces from Delaware and West Virginia, in this study and from Arkansas, Georgia, Florida, Minnesota, Oklahoma and Utah previously. [PUBLICATION ABSTRACT] To validate the distribution of a poultry litter-specific marker gene in faecally contaminated environmental waters of an intensive poultry litter rearing region. A TaqMan(®)-based qPCR assay for Brevibacterium sp. LA35 16S rRNA (LA35 gene), which was previously shown to be associated with poultry litter and faeces, was tested on 126 nontarget faecal samples and 28 poultry litter and faecal samples. The TaqMan assay was sensitive (76%) and specific (100%) to the LA35 gene and exhibited a detection limit for poultry litter in water samples that is sufficiently low (2.5 × 10(-2) mg litter l(-1)) to be applicable for environmental monitoring. The LA35 gene was detected in 43% of water samples (n = 30) collected in an intensive poultry rearing region of West Virginia which drains to the Chesapeake Bay. The poultry-specific TaqMan qPCR method for the LA35 gene is more specific than previously published methods and can be used to identify regions impacted by poultry rearing activities. The LA35 gene appears to have a broad geographical distribution as it has been found in poultry litter and faeces from Delaware and West Virginia, in this study and from Arkansas, Georgia, Florida, Minnesota, Oklahoma and Utah previously. AIMTo validate the distribution of a poultry litter-specific marker gene in faecally contaminated environmental waters of an intensive poultry litter rearing region.METHODS AND RESULTSA TaqMan(®)-based qPCR assay for Brevibacterium sp. LA35 16S rRNA (LA35 gene), which was previously shown to be associated with poultry litter and faeces, was tested on 126 nontarget faecal samples and 28 poultry litter and faecal samples. The TaqMan assay was sensitive (76%) and specific (100%) to the LA35 gene and exhibited a detection limit for poultry litter in water samples that is sufficiently low (2.5 × 10(-2) mg litter l(-1)) to be applicable for environmental monitoring. The LA35 gene was detected in 43% of water samples (n = 30) collected in an intensive poultry rearing region of West Virginia which drains to the Chesapeake Bay.CONCLUSIONSThe poultry-specific TaqMan qPCR method for the LA35 gene is more specific than previously published methods and can be used to identify regions impacted by poultry rearing activities.SIGNIFICANCE AND IMPACT OF THE STUDYThe LA35 gene appears to have a broad geographical distribution as it has been found in poultry litter and faeces from Delaware and West Virginia, in this study and from Arkansas, Georgia, Florida, Minnesota, Oklahoma and Utah previously. Aim To validate the distribution of a poultry litter‐specific marker gene in faecally contaminated environmental waters of an intensive poultry litter rearing region. Methods and Results A TaqMan®‐based qPCR assay for Brevibacterium sp. LA35 16S rRNA (LA35 gene), which was previously shown to be associated with poultry litter and faeces, was tested on 126 nontarget faecal samples and 28 poultry litter and faecal samples. The TaqMan assay was sensitive (76%) and specific (100%) to the LA35 gene and exhibited a detection limit for poultry litter in water samples that is sufficiently low (2·5 × 10−2 mg litter l−1) to be applicable for environmental monitoring. The LA35 gene was detected in 43% of water samples (n = 30) collected in an intensive poultry rearing region of West Virginia which drains to the Chesapeake Bay. Conclusions The poultry‐specific TaqMan qPCR method for the LA35 gene is more specific than previously published methods and can be used to identify regions impacted by poultry rearing activities. Significance and Impact of the Study The LA35 gene appears to have a broad geographical distribution as it has been found in poultry litter and faeces from Delaware and West Virginia, in this study and from Arkansas, Georgia, Florida, Minnesota, Oklahoma and Utah previously. |
Author | Lipscomb, E. Weidhaas, J. |
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Cites_doi | 10.1007/s00253-006-0333-6 10.1016/j.watres.2007.06.037 10.1128/AEM.02555-10 10.2134/jeq1997.00472425002600040026x 10.1128/AEM.05734-11 10.1007/s00253-012-4469-2 10.1111/j.1365-2672.2010.04666.x 10.1128/AEM.00019-08 10.1128/AEM.00110-10 10.1093/nar/gkm864 10.1586/14737159.5.2.209 10.1128/AEM.02350-09 10.1073/pnas.82.20.6955 10.1016/j.watres.2007.05.033 10.1093/nar/17.19.7843 10.1128/AEM.00462-09 10.1111/j.0902-0055.2004.00135.x 10.1373/clinchem.2008.112797 10.1128/AEM.00895-12 10.1128/AEM.07923-11 10.1099/00221287-148-1-257 |
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Keywords | Litter Applied microbiology Poultry poultry litter Brevibacteriaceae Brevibacterium sp Method Rivers Surveillance Microbial source tracking LA35 Brevibacterium Bacteria Actinomycetes qPCR Potomac River Basin Brevibacterium sp. LA35 microbial source tracking |
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To validate the distribution of a poultry litter‐specific marker gene in faecally contaminated environmental waters of an intensive poultry litter rearing... To validate the distribution of a poultry litter-specific marker gene in faecally contaminated environmental waters of an intensive poultry litter rearing... Aim To validate the distribution of a poultry litter-specific marker gene in faecally contaminated environmental waters of an intensive poultry litter rearing... AIMTo validate the distribution of a poultry litter-specific marker gene in faecally contaminated environmental waters of an intensive poultry litter rearing... |
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SubjectTerms | Animals Biological and medical sciences Brevibacterium Brevibacterium - genetics Brevibacterium - isolation & purification Brevibacterium sp. LA35 Environmental Monitoring - methods Feces - microbiology Fundamental and applied biological sciences. Psychology microbial source tracking Microbiology Polymerase Chain Reaction - methods Potomac River Basin Poultry - microbiology poultry litter qPCR Rivers - microbiology RNA, Ribosomal, 16S - genetics Water Pollutants - isolation & purification West Virginia |
Title | A new method for tracking poultry litter in the Potomac Basin headwaters of West Virginia |
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