Characterization of papillomavirus polypeptides from bovine cutaneous fibropapillomas

• Published in: Journal of general virology Vol. 69; no. 11; pp. 2919 - 2924
• Main Authors: Della Torre, G, Muttini, M, Ballinari, D, Ferrari, G, Pierotti, M.A, Della Porta, G
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.11.1988; Society for General Microbiology
• Subjects: Animals; Biological and medical sciences; Blotting, Western; Bovidae; Bovine papillomavirus; Cattle; Cattle Diseases - microbiology; Electrophoresis, Gel, Two-Dimensional; fibroma; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Immunobiology; Isoelectric Point; Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation; Molecular Weight; Papilloma - microbiology; Papilloma - veterinary; Papillomaviridae - analysis; Papillomaviridae - immunology; polypeptides; Skin Neoplasms - microbiology; Skin Neoplasms - veterinary; Viral Proteins - immunology; Viral Proteins - isolation & purification; Papillomavirus; Virus; Bovine papillomavirus; Biochemical analysis; Gel electrophoresis; Immunological investigation; Polypeptide; Papilloma; Papovaviridae; Capsid
• DOI: 10.1099/0022-1317-69-11-2919
• ISSN: 0022-1317

1 Division of Experimental Oncology A, and 2 Division of Experimental Oncology D, Istituto Nazionale per lo Studio e la Cura dei Tumori, Via G. Venezian 1, 20133 Milan, Italy Virions of bovine papilloma virus (BPV) were isolated from a pool of cutaneous bovine fibropapillomas and purified by CsCl gradient centrifugation. SDS-PAGE revealed several polypeptides with an M r ranging from 76K to 19K. Western blot analysis of the viral isolate identified additional polypeptides when a rabbit anti-BPV serum was used, but only the main capsid component of 57K when a rabbit antiserum raised against human papillomavirus was used. The viral preparation was then 125 I-labelled and further purified by gel filtration. SDS-PAGE of immunoprecipitates of the anti-BPV serum with different fractions from the chromatographic column revealed the polypeptides of 76K, 57K and 28K to be viral structural components. The 28K polypeptide, not previously characterized, was shown to be composed of several molecular forms, migrating over a pH range of 3.5 to 4.6 when analysed by two-dimensional PAGE. Following SDS-PAGE performed under non-reducing conditions, the 28K and 76K polypeptides and the main capsid component of 57K appeared to be linked by disulphide bridges to form hetero- or homopolymers. Keywords: BPV, fibropapillomas, capsid proteins Received 8 April 1988; accepted 25 July 1988.