Liposome-Mediated Combinatorial Cytokine Gene Therapy Induces Localized Synergistic Immunosuppression and Promotes Long-Term Survival of Cardiac Allografts

• Published in: The Journal of immunology (1950) Vol. 174; no. 11; pp. 6983 - 6992
• Main Authors: Furukawa, Hiroshi, Oshima, Kiyohiro, Tung, Thomas, Cui, Guanggen, Laks, Hillel, Sen, Luyi
• Format: Journal Article
• Language: English
• Published: United States Am Assoc Immnol 01.06.2005
• Subjects: Adjuvants, Immunologic - administration & dosage; Adjuvants, Immunologic - biosynthesis; Adjuvants, Immunologic - genetics; Animals; Cell Migration Inhibition; Cytokines - administration & dosage; Cytokines - biosynthesis; Cytokines - genetics; Cytotoxicity, Immunologic - genetics; Cytotoxicity, Immunologic - immunology; Drug Combinations; Genetic Therapy - adverse effects; Genetic Therapy - methods; Genetic Vectors; Graft Enhancement, Immunologic - adverse effects; Graft Enhancement, Immunologic - methods; Graft Rejection - genetics; Graft Rejection - immunology; Graft Rejection - prevention & control; Graft Survival - genetics; Graft Survival - immunology; Heart Transplantation - adverse effects; Heart Transplantation - immunology; Heart Transplantation - pathology; Humans; Immunosuppressive Agents - administration & dosage; Immunosuppressive Agents - adverse effects; Interleukin-10 - administration & dosage; Interleukin-10 - biosynthesis; Interleukin-10 - genetics; Interleukin-4 - administration & dosage; Interleukin-4 - biosynthesis; Interleukin-4 - genetics; Liposomes; Rabbits; T-Lymphocyte Subsets - cytology; T-Lymphocyte Subsets - immunology; Th1 Cells - immunology; Th1 Cells - metabolism; Th2 Cells - immunology; Th2 Cells - metabolism; Transplantation, Heterotopic

Localized gene transfer has the potential to introduce immunosuppressive molecules only into the transplanted allograft, which would limit systemic side effects, and prolong allograft survival. However, an applicable gene transfer strategy is not available, and the feasible therapeutic gene(s) has n...