β-Cyanoalanine Synthase Is a Mitochondrial Cysteine Synthase-like Protein in Spinach and Arabidopsis

• Published in: Plant physiology (Bethesda) Vol. 123; no. 3; pp. 1163 - 1171
• Main Authors: Yves Hatzfeld, Akiko Maruyama, Ahlert Schmidt, Noji, Masaaki, Kimiharu Ishizawa, Saito, Kazuki
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Physiologists 01.07.2000
• Subjects: Agronomy. Soil science and plant productions; Amino acids; Antibodies; Arabidopsis - enzymology; Arabidopsis - genetics; Biochemical Processes and Macromolecular Structures; Biological and medical sciences; Complementary DNA; Cyanides; Cysteine Synthase - genetics; Cysteine Synthase - metabolism; Economic plant physiology; Enzymes; Escherichia coli - genetics; Escherichia coli - metabolism; Evolution, Molecular; Fundamental and applied biological sciences. Psychology; Immunoblotting; Isoenzymes - genetics; Isoenzymes - metabolism; Lyases - genetics; Lyases - metabolism; Metabolism; Mitochondria; Mitochondria - enzymology; Mitochondria - genetics; Nutrition. Photosynthesis. Respiration. Metabolism; Phylogeny; Plant physiology and development; Protein isoforms; Proteins; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Spinach; Spinacia oleracea - enzymology; Spinacia oleracea - genetics; Substrate Specificity; Sulfides; Cysteine; L-3-Cyanoalanine synthase; Enzyme; Isozyme; Interspecific comparison; Nomenclature; Lyases; Identification; Metabolic pathway; Spinacia oleracea; Chenopodiaceae; Arabidopsis thaliana; Mitochondria; Cruciferae; Aminoacid; Dicotyledones; Cysteine synthase; DNA; Angiospermae; Carbon-sulfur lyases; Spermatophyta; Experimental plant; Localization; Carbon-oxygen lyases

β-Cyano-alanine synthase (CAS; EC 4.4.1.9) plays an important role in cyanide metabolism in plants. Although the enzymatic activity of β-cyano-Ala synthase has been detected in a variety of plants, no cDNA or gene has been identified so far. We hypothesized that the mitochondrial cysteine synthase (...