HIFI-α activation underlies a functional switch in the paradoxical role of Ezh2/PRC2 in breast cancer

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 113; no. 26; pp. E3735 - E3744
• Main Authors: Mahara, Sylvia, Lee, Puay Leng, Feng, Min, Tergaonkar, Vinay, Chng, Wee Joo, Yu, Qiang
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 28.06.2016
• Series: PNAS Plus
• Subjects: Biological Sciences; Breast Neoplasms - genetics; Breast Neoplasms - metabolism; Breast Neoplasms - physiopathology; Cell Line, Tumor; Cell Proliferation; Enhancer of Zeste Homolog 2 Protein - genetics; Enhancer of Zeste Homolog 2 Protein - metabolism; Humans; Hypoxia-Inducible Factor 1, alpha Subunit - genetics; Hypoxia-Inducible Factor 1, alpha Subunit - metabolism; PNAS Plus; Polycomb Repressive Complex 2 - genetics; Polycomb Repressive Complex 2 - metabolism; hypoxia; breast cancer; PRC2; FOXM1; EZH2
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.1602079113

Despite the established oncogenic function of Polycomb repressive complex 2 (PRC2) in human cancers, its role as a tumor suppressor is also evident; however, the mechanism underlying the regulation of the paradoxical functions of PRC2 in tumorigenesis is poorly understood. Here we show that hypoxia-inducible factor 1, α-subunit (HIFI-α) is a crucial modulator of PRC2 and enhancer of zeste 2 (EZH2) function in breast cancer. Interrogating the genomic expression of breast cancer indicates high HIF1A activity correlated with high EZH2 expression but low PRC2 activity in triple-negative breast cancer compared with other cancer subtypes. In the absence of HIFIA activation, PRC2 represses the expression of matrix metalloproteinase genes (MMPs) and invasion, whereas a discrete Ezh2 complexed with Forkhead box M1 (FoxM1) acts to promote the expression of MMPs. HIF1-α induction upon hypoxia results in PRC2 inactivation by selective suppression of the expression of suppressor of zeste 12 protein homolog (SUZ12) and embryonic ectoderm development (EED), leading to a functional switch toward Ezh2/FoxM1-dependent induction of the expression of MMPs and invasion. Our study suggests a tumor-suppressive function of PRC2, which is restricted by HIF1-α, and an oncogenic function of Ezh2, which cooperates with FoxM1 to promote invasion in triple-negative breast cancer.