Analyzing rare mutations in metagenomes assembled using long and accurate reads

• Published in: Genome research Vol. 32; no. 11-12; pp. 2119 - 2133
• Main Authors: Fedarko, Marcus W., Kolmogorov, Mikhail, Pevzner, Pavel A.
• Format: Journal Article
• Language: English
• Published: United States Cold Spring Harbor Laboratory Press 01.11.2022
• Subjects: Bacteria - genetics; Genomes; Metagenome; Metagenomics; Method; Mutation

The advent of long and accurate “HiFi” reads has greatly improved our ability to generate complete metagenome-assembled genomes (MAGs), enabling “complete metagenomics” studies that were nearly impossible to conduct with short reads. In particular, HiFi reads simplify the identification and phasing...