A Rapid Point-of-Care Assay for Cysticercosis Antigen Detection in Urine Samples
We report a proof-of-concept study using a dipstick assay to detect Taenia solium antigen in urine samples of 30 patients with subarachnoid neurocysticercosis and 10 healthy control subjects. Strips were read in blind by two readers. The assay detected antigen in 29 of 30 cases and was negative in a...
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| Published in | The American journal of tropical medicine and hygiene Vol. 108; no. 3; pp. 578 - 580 |
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| Main Authors | , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
Institute of Tropical Medicine
01.03.2023
The American Society of Tropical Medicine and Hygiene |
| Subjects | |
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| Abstract | We report a proof-of-concept study using a dipstick assay to detect
Taenia solium
antigen in urine samples of 30 patients with subarachnoid neurocysticercosis and 10 healthy control subjects. Strips were read in blind by two readers. The assay detected antigen in 29 of 30 cases and was negative in all 10 control samples. Although this study was performed in samples from individuals with subarachnoid neurocysticercosis who likely had high circulating antigen levels, it provides the proof of concept for a functional urine antigen point-of-care assay that detects viable cysts. Such an assay could serve to support a clinical diagnosis of suspect neurocysticercosis or to identify patients at risk of developing severe disease in areas where medical resources are limited, providing evidence to refer these individuals for imaging and specialized care as needed. |
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| AbstractList | We report a proof-of-concept study using a dipstick assay to detect Taenia solium antigen in urine samples of 30 patients with subarachnoid neurocysticercosis and 10 healthy control subjects. Strips were read in blind by two readers. The assay detected antigen in 29 of 30 cases and was negative in all 10 control samples. Although this study was performed in samples from individuals with subarachnoid neurocysticercosis who likely had high circulating antigen levels, it provides the proof of concept for a functional urine antigen point-of-care assay that detects viable cysts. Such an assay could serve to support a clinical diagnosis of suspect neurocysticercosis or to identify patients at risk of developing severe disease in areas where medical resources are limited, providing evidence to refer these individuals for imaging and specialized care as needed. We report a proof-of-concept study using a dipstick assay to detect Taenia solium antigen in urine samples of 30 patients with subarachnoid neurocysticercosis and 10 healthy control subjects. Strips were read in blind by two readers. The assay detected antigen in 29 of 30 cases and was negative in all 10 control samples. Although this study was performed in samples from individuals with subarachnoid neurocysticercosis who likely had high circulating antigen levels, it provides the proof of concept for a functional urine antigen point-of-care assay that detects viable cysts. Such an assay could serve to support a clinical diagnosis of suspect neurocysticercosis or to identify patients at risk of developing severe disease in areas where medical resources are limited, providing evidence to refer these individuals for imaging and specialized care as needed.We report a proof-of-concept study using a dipstick assay to detect Taenia solium antigen in urine samples of 30 patients with subarachnoid neurocysticercosis and 10 healthy control subjects. Strips were read in blind by two readers. The assay detected antigen in 29 of 30 cases and was negative in all 10 control samples. Although this study was performed in samples from individuals with subarachnoid neurocysticercosis who likely had high circulating antigen levels, it provides the proof of concept for a functional urine antigen point-of-care assay that detects viable cysts. Such an assay could serve to support a clinical diagnosis of suspect neurocysticercosis or to identify patients at risk of developing severe disease in areas where medical resources are limited, providing evidence to refer these individuals for imaging and specialized care as needed. We report a proof-of-concept study using a dipstick assay to detect Taenia solium antigen in urine samples of 30 patients with subarachnoid neurocysticercosis and 10 healthy control subjects. Strips were read in blind by two readers. The assay detected antigen in 29 of 30 cases and was negative in all 10 control samples. Although this study was performed in samples from individuals with subarachnoid neurocysticercosis who likely had high circulating antigen levels, it provides the proof of concept for a functional urine antigen point-of-care assay that detects viable cysts. Such an assay could serve to support a clinical diagnosis of suspect neurocysticercosis or to identify patients at risk of developing severe disease in areas where medical resources are limited, providing evidence to refer these individuals for imaging and specialized care as needed. |
| Author | Castillo, Yesenia O’Neal, Seth E. Bustos, Javier A. Marin, Yazmin Toribio, Luz Handali, Sukwan Garcia, Hector H. Perez, Erika |
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| Copyright | Copyright Institute of Tropical Medicine Mar 2023 The American Society of Tropical Medicine and Hygiene 2023 |
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| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 Authors’ addresses: Luz Toribio and Javier A. Bustos, Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Peru, and Cysticercosis Unit, Instituto Nacional de Ciencias Neurologicas, Lima, Peru, E-mails: luz.toribio.salazar@upch.pe and javier.bustos.p@upch.pe. Sukwan Handali, Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, GA, E-mail: ahi0@cdc.gov. Yazmin Marin and Erika Perez, Cysticercosis Unit, Instituto Nacional de Ciencias Neurologicas, Lima, Peru, E-mails: yazmin.marin@upch.pe and perez.paredes.e@gmail.com. Yesenia Castillo, Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Peru, and Parasite Immunology Laboratory, Universidad Peruana Cayetano Heredia, Lima, Peru, E-mail: yesenia.castillo.b@upch.pe. Seth E. O’Neal, School of Public Health, Oregon Health & Sciences University-Portland State University, Portland, OR, E-mail: natseth7@gmail.com. Hector H. Garcia, Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Peru, Cysticercosis Unit, Instituto Nacional de Ciencias Neurologicas, Lima, Peru, Asociacion Bene fica PRISMA, Lima, Peru, and Department of International Health, Johns Hopkins School of Public Health, Baltimore, MD, E-mail: hgarcia@jhsph.edu. |
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| References | Garcia (b6) 2012; 17 Castillo (b7) 2009; 80 Toribio (b10) 2019; 100 Mwape (b8) 2011; 105 Garcia (b2) 2020; 33 Abanto (b11) 2021; 105 Cantey (b1) 2014; 90 Bustos (b4) 2021; 427 Rodriguez (b5) 2012; 106 McCleery (b9) 2020; 103 White (b3) 2018; 66 Paredes (b12) 2016; 166 |
| References_xml | – volume: 33 start-page: e00085-19 year: 2020 ident: b2 article-title: Taenia solium cysticercosis and its impact in neurological disease – volume: 66 start-page: 1159 year: 2018 ident: b3 article-title: Diagnosis and treatment of neurocysticercosis: 2017 clinical practice guidelines by the Infectious Diseases Society of America (IDSA) and the American Society of Tropical Medicine and Hygiene (ASTMH) – volume: 105 start-page: 574 year: 2011 ident: b8 article-title: Field evaluation of urine antigen detection for diagnosis of Taenia solium cysticercosis – volume: 103 start-page: 1125 year: 2020 ident: b9 article-title: Population screening for urine antigens to detect asymptomatic subarachnoid neurocysticercosis: a pilot study in northern Peru – volume: 100 start-page: 327 year: 2019 ident: b10 article-title: Detection of Taenia solium DNA in the urine of neurocysticercosis patients – volume: 106 start-page: 286 year: 2012 ident: b5 article-title: Immunological and molecular diagnosis of cysticercosis – volume: 17 start-page: 1014 year: 2012 ident: b6 article-title: Neurocysticercosis: is serology useful in the absence of brain imaging? – volume: 105 start-page: 176 year: 2021 ident: b11 article-title: Mortality in parenchymal and subarachnoid neurocysticercosis – volume: 90 start-page: 805 year: 2014 ident: b1 article-title: Neglected parasitic infections in the United States: cysticercosis – volume: 427 start-page: 117527 year: 2021 ident: b4 article-title: Neurocysticercosis: a frequent cause of seizures, epilepsy, and other neurological morbidity in most of the world – volume: 80 start-page: 379 year: 2009 ident: b7 article-title: Urine antigen detection for the diagnosis of human neurocysticercosis – volume: 166 start-page: 37 year: 2016 ident: b12 article-title: Anti-Taenia solium monoclonal antibodies for the detection of parasite antigens in body fluids from patients with neurocysticercosis |
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| Snippet | We report a proof-of-concept study using a dipstick assay to detect
Taenia solium
antigen in urine samples of 30 patients with subarachnoid neurocysticercosis... We report a proof-of-concept study using a dipstick assay to detect Taenia solium antigen in urine samples of 30 patients with subarachnoid neurocysticercosis... |
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| SubjectTerms | Animals Antigens Antigens, Helminth Bioassays Cysticercosis Enzyme-Linked Immunosorbent Assay - methods Humans Neurocysticercosis - diagnosis Parasites Parasitic diseases Point of care testing Point-of-Care Systems Short Report Taenia solium Urine |
| Title | A Rapid Point-of-Care Assay for Cysticercosis Antigen Detection in Urine Samples |
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